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OPTIMIZATION OF ELECTROPHORESIS GELS FOR RIS ANALYSIS

Award Information

Agency:
Department of Health and Human Services
Branch:
N/A
Award ID:
16673
Program Year/Program:
1991 / SBIR
Agency Tracking Number:
16673
Solicitation Year:
N/A
Solicitation Topic Code:
N/A
Solicitation Number:
N/A
Small Business Information
ATOM SCIENCES, INC.
ATOM SCIENCES, INC. 114 RIDGEWAY CTR OAK RIDGE, TN 37830 8810
View profile »
Woman-Owned: No
Minority-Owned: No
HUBZone-Owned: No
 
Phase 1
Fiscal Year: 1991
Title: OPTIMIZATION OF ELECTROPHORESIS GELS FOR RIS ANALYSIS
Agency: HHS
Contract: N/A
Award Amount: $49,095.00
 

Abstract:

GIVEN A FAST, SENSITIVE DETECTION METHOD, A LARGE NUMBER OF STABLE ISOTOPES COULD BE USED TO LABEL DNA, THEREBY MULTIPLEXING THE SEPARATION PROCESS, AND PROVIDING A NEW, MUCH FASTER PROCEDURE FOR LOCALIZING DNA AFTER ELECTROPHORESIS. SPUTTER-INITIATED RESONANCE IONIZATION SPECTROSCOPY (SIRIS) CAN BE USED TO MEASURE SUBATTOMOLE QUANTITIES OF ISOTOPE-LABELED DNA WITH EXCELLENT LATERAL AND MASS RESOLUTION. SIRIS ANALYSIS HAS THE POTENTIAL OF ORDERS-OF-MAGNITUDE IMPROVEMENT IN THE SPEED OF DNA SEQUENCING, BUT REQUIRES ELECTROPHORESIS GELS IN WHICH THE SEPARATED DNA FRAGMENTS ARE CONCENTRATED AT THE GEL SURFACE AND IN BANDS OF ABOUT 100 FM WIDTH. THE OBJECTIVE IS TO DEVELOP ANALYSIS METHODS FOR ELECTROPHORESIS GELS THAT CAN LOCALIZE SUBFEMTOMOLE CONCENTRATIONS OF ISOTOPE-LABELED DNA WITH MICROMETER LATERAL AND NANOMETER DEPTH RESOLUTION LEADING TO IMPROVED ELECTROPHORESIS MATERIALS AND PROCESSES THAT BEST MATCH THE ULTRAHIGH ELEMENT SENSITIVITY AVAILABLE WITH SIRIS. WE WILL OPTIMIZE SIRIS TO DETERMINE THE SPATIAL DISTRIBUTION OF ISOTOPE-LABELED DNA BANDS IN ULTRATHIN PAGE SAMPLES AND EXPLOIT OPTIMIZATION OF GEL PARAMETERS SUCH AS CASTING PROCEDURES, THICKNESS, MATRIX MODIFIERS, TEMPERATURE SHRINKING RATIOS, DRYING PROCESSES LEAD TO HIGH RESOLUTION GEL ELECTROPHORESIS WITH SURFACE ENHANCED DNA DISTRIBUTION BY THE END OF PHASE II.

Principal Investigator:

Arlinghaus, Heinrich F
Principal Investigator
6154831113

Business Contact:

1 r43 hg00532-01
Small Business Information at Submission:

Atom Sciences, Inc
114 Ridgeway Center Oak Ridge, TN 37830

EIN/Tax ID:
DUNS: N/A
Number of Employees: N/A
Woman-Owned: No
Minority-Owned: No
HUBZone-Owned: No