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Optimizing Electrophoresis Gels for Rimp Analysis

Award Information

Agency:
Department of Health and Human Services
Branch:
N/A
Award ID:
24939
Program Year/Program:
1995 / SBIR
Agency Tracking Number:
24939
Solicitation Year:
N/A
Solicitation Topic Code:
N/A
Solicitation Number:
N/A
Small Business Information
ATOM SCIENCES, INC.
ATOM SCIENCES, INC. 114 RIDGEWAY CTR OAK RIDGE, TN 37830 8810
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Woman-Owned: No
Minority-Owned: No
HUBZone-Owned: No
 
Phase 2
Fiscal Year: 1995
Title: Optimizing Electrophoresis Gels for Rimp Analysis
Agency: HHS
Contract: 1 R43 CA66525-1,
Award Amount: $500,000.00
 

Abstract:

This project uses sequencing by hybridization to develop a new method to detect mutations,allowing disease diagnoses in selected genes from a large number of individuals, in a faster, moreeconomical and more comprehensive way than can currently be accomplished. Stable isotopes is utilizedas DNA labels to demonstrate the basic advantages of such labels and to show the multiplexingfeasibility. We have demonstrated that stable isotopes function as both DNA and oligonucleotide labelsand that resonance ionization spectroscopy can offer a very fast method for the detection ofsurface-bound DNA with a high degree of sensitivity, selectivity, spatial resolution, and analysis speed.In Phase I, we evaluate two potentially viable detection techniques for rapid genome diagnosis usinggenosensor matrices: sputter-initiated resonance ionization spectroscopy (SIRIS) and laser atomizationRIS (LARIS). Measurements are made on quartz surfaces, onto which oligonucleotide sequences havebeen attached, to identify positively hybridized and unhybridized sites and to determine the sensitivity,efficiency, background, spatial resolution, and speed - and the interrelationships and trade-offs amongthem.

Principal Investigator:

Heinrich Arlinghaus
6154831113

Business Contact:

Small Business Information at Submission:

Atom Sciences, Inc.
114 Ridgeway Center Oak Ridge, TN 37830

EIN/Tax ID:
DUNS: N/A
Number of Employees: N/A
Woman-Owned: No
Minority-Owned: No
HUBZone-Owned: No