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A NOVEL ELISA FOR THE DETECTION OF ANTIBODIES TO HIV-1

Award Information

Agency:
Department of Health and Human Services
Branch:
N/A
Award ID:
18942
Program Year/Program:
1992 / SBIR
Agency Tracking Number:
18942
Solicitation Year:
N/A
Solicitation Topic Code:
N/A
Solicitation Number:
N/A
Small Business Information
Aug, Inc.
1440 Canal Street New Orleans, LA 70112
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Woman-Owned: No
Minority-Owned: No
HUBZone-Owned: No
 
Phase 1
Fiscal Year: 1992
Title: A NOVEL ELISA FOR THE DETECTION OF ANTIBODIES TO HIV-1
Agency: HHS
Contract: N/A
Award Amount: $50,000.00
 

Abstract:

THE LONG-TERM OBJECTIVE OF THIS CLINICAL RESEARCH IS TO FURTHER DEVELOP A NOVEL ELISA FOR THE DETECTION OF ANTIBODIES TO HIV-1 ENVELOPE GLYCOPROTEINS. THE UTILIZATION OF NATIVE ENVLOPE HIV-1 GLYCOPROTEINS OFFERS THE POSSIBILITY OF DETECTING ANTIBODIES IN PATIENT ERA TO A BROADER RANGE OF ANTIGENIC DETERMINANTS INCLUDING CONFORMATIONAL EPITOPES. THE ENHANCED SENSITIVITY OF THIS NOVEL ELISA WILL BE EVALUATED FOR POTENTIAL PROGNOSTIC CLINICAL UTILITY IN THE EARLY AND MORE EFFECTIVE TREATMENT OF THE HIV-1 INFECTION. A NOVEL METHOD THAT GREATLY SIMPLIFIES THE PREPARATION OF SOLID-PHASE HIV-1 ENVELOPE GLYCOPROTEINS FOR USE IN THIS ELISA HAS BEEN DEVELOPED. THIS METHOD UTILIZES CONCANAVALIN A ABSORBED TO WELLS OF MICROTITER PLATES TO AFFINITY IMMOBILIZE DETERGENT SOLUBILIZED VIRAL GLYCOPROTEINS RELEASED IN CULTURE FLUIDS OF HIV-1 INFECTED CELL LINES GROWN IN SERUM-FREE MEDIUM. SINCE CONCANAVALIN A HAS A GENERAL SPECIFICITY FOR A VARIETY OF GLYCOPROTEINS CONTAINING MANNOSYL RESIDUES, IT SHOULD BE POSSIBLE TO DEVELOP ELISA'S THAT DETECT GLYCOPROTEIN-SPECIFIC ANTIBOIDES TO A DIVERSITY OF ENVELOPED VIRUSES, INCLUDING OTHER HUMAN AND ANIMAL RETROVIRUSES.

Principal Investigator:

Craig Cohen
5045234226

Business Contact:

Small Business Information at Submission:

Aug, Inc.
1440 Canal Street New Orleans, LA 70112

EIN/Tax ID:
DUNS: N/A
Number of Employees: N/A
Woman-Owned: No
Minority-Owned: No
HUBZone-Owned: No