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Ovalbumin locus BACs for expression of biotherapeutics

Award Information

Agency:
Department of Health and Human Services
Branch:
N/A
Award ID:
66488
Program Year/Program:
2003 / SBIR
Agency Tracking Number:
GM068200
Solicitation Year:
N/A
Solicitation Topic Code:
N/A
Solicitation Number:
N/A
Small Business Information
AviGenics Inc.
111 Riverbend Rd. 411 MASSACHUSETTS AVENUE Athens, GA 30605 2613
View profile »
Woman-Owned: No
Minority-Owned: No
HUBZone-Owned: No
 
Phase 1
Fiscal Year: 2003
Title: Ovalbumin locus BACs for expression of biotherapeutics
Agency: HHS
Contract: 1R43GM068200-01
Award Amount: $81,461.00
 

Abstract:

DESCRIPTION (provided by applicant): With a daily production capacity of two grams of protein per egg, the chicken ovalbumin gene locus has the potential to synthesize large amounts of biopharmaceutical proteins into the eggs of transgenic hens. This phase I SBIR project will test the feasibility of using large expression constructs, based on the complete 200 kilobase chicken ovalbumin gene locus, to drive efficient expression of biopharmaceutical proteins including humanized monoclonal antibodies. The longer-term objectives of the program are to use these constructs in the creation of transgenic hens, in phase II that will produce biopharmaceutical proteins at mg/ml concentration in egg white. In this phase I proposal, we plan to create a complete 200 kilobase ovalbumin gene from two overlapping bacterial artificial chromosomes (BAC) by utilizing recA-assisted restriction endonuclease (RARE) cleavage techniques. Using similar techniques, we will then insert reporter and biopharmaceutical genes into various locations within the transcribed region of the ovalbumin gene. Finally, we will assay protein expression from these constructs using in vitro and in vivo assays.

Principal Investigator:

Mark C. Leavitt
7062271170
LEAVITT@AVIGENICS.COM

Business Contact:


7062271170
Small Business Information at Submission:

AVIGENICS, INC.
AVIGENICS, INC. GEORGIA BIOBUSINESS CENTER ATHENS, GA 30605

EIN/Tax ID: 943244869
DUNS: N/A
Number of Employees: N/A
Woman-Owned: No
Minority-Owned: No
HUBZone-Owned: No