The long-term gosl of this research is to construct and evaluate a noninvasive device that will assess the functional integrity of hypothermic perfused kidneys prior to transplant. the device is based on measurements of changes in mitochondrial electron-transport protein-oxidation states in the… More

The long-term goal of this research is to develop quantitative imaging methods for tissue bioenergetic studies. toward this end, it is proposed to construct and evaluate an instrument that will image the response of mitochondrial metabolites to ischemia and hypoxia in rabbit hearts in situ.… More

The long term goal of this research is to develop an imaging system to evaluate viability of vascularized skin grafts. towards this end it is proposed to construct and evaluate a digitized imager that will visually display two parameters that correlate with skin graft viability. the first technique… More

The long-term goal of this research is to develop optical instruments and methods for measuring and imaging tissue temperature. in phase i, techniques will be developed that compute temperature readings from near-infrared (nir) absorption bands of water. the effect of interference on such… More

The long-term goal of this research is to develop quantitative optical imaging methods for bioenergetic studies of tissue. methods will be developed for the optical determination of tissue or circulatory oxygen concentration based on palladium porphyrin phosphorescence quenching by collisional… More

This research project involves the development of optical methods to quantify the total available oxygen content of opicardial tissue. epicardial oxygen is found in three major pools-as oxygen dissolved in aqueous media, as oxygen bound to hemoblobin and as oxygen bound to myoglobin. in this… More

This research will develop an optical instrument to aid in the noninvasive evaluation of skinaffected by injury, surgical procedures and/or disease. The instrument will use characteristics of lightabsorption by hemoglobin species to delineate skin regions compromised by ischemia, hypoxia ornecrosis.… More

We will develop a new approach to the evaluation of microregional circulation. This research is based on the working hypothesis that fluorescent microspheres can be spatially located by fluorescence imaging of organ serial sections. The project will develop instrumentation and procedures to evaluate… More

The long term goal is to develop noninvasive optical instruments and methods for assessing the functional integrity of cold-stored livers prior to transplant. In hypothermically-stored livers, areas of dysfunctional oxidative metabolism can be detected noninvasively using spectrophotometric methods.… More

This research will develop an instrument to aid in the rapid, non-invasive measurement of ulcer sizhealing. The device will quantitative wound volume using a structured laser light, with optical imagcurve-fitting methodologies. This research is based on the hypothesis that when structured light… More

We will develop low cost and accurate methods for regional flow measurements in tissues. This reseaon the working hypothesis that fluorescent microspheres in tissue digests can be accurately and rapifluorescence flow system. The project will develop instrumentation and evaluate procedures to… More

DESCRIPTION (Verbatim From Applicant's Abstract): The goal of this project is to develop a fully automated method and instrument for high-resolution three-dimensional mapping of regional blood flow distribution in tumors. This research is based upon the working hypothesis… More

This research involves developing procedures to determine regional flow in tissues and organs at high resolution. Current methods for regional flow determinations are limited by microsphere size and counting statistics. This study tests the hypothesis that fluorescently-labeled antibodies, serving… More

DESCRIPTION (provided by applicant): In order for a tumor to grow, it must obtain nutrients and oxygen from its host and continuously evolve new vasculature to deliver supplies to its multiplying cells. Exciting current research studies of interventions that reduce or reverse malignant growth of… More

DESCRIPTION (provided by applicant): In order for a tumor to grow, it must obtain nutrients and oxygen from its host and continuously evolve new vasculature to deliver supplies to its multiplying cells. Exciting current research studies of interventions that reduce or reverse malignant growth of… More

DESCRIPTION (provided by applicant): This research involves the development of a method to quantify regional flow in tissues using automated instrumental procedures. Phase I results have demonstrated that fluorescent antibodies, specific to antigens in capillary endothelium, can be spatially… More

DESCRIPTION (provided by applicant): In order to assure the physiological status of small experimental animals, pulmonary and circulatory functions must be monitored accurately and frequently. Arterial blood-gas measurements are critical for this monitoring, however, repeated measurements can affect… More

DESCRIPTION (provided by applicant): Locating fluorescently-tagged cells in whole organisms is important to the development of gene therapy, stem cell therapy delivery methods, and for drug research. This project will develop an intelligent microtome to identify fluorescently labeled cells in whole… More

DESCRIPTION (provided by applicant): Regional blood flow distributions in organs of experimental animals can be measured effectively by combining fluorescent microsphere methods with imaging cryomicrotomy. The number of flow measurements that can be made i n a single animal is currently limited… More

DESCRIPTION (provided by applicant): Fluorescent tagging of cells using dyes, fluorescent proteins, and quantum dots forms the basis for many powerful analytical methods in modern biology. Locating these cells within entire animals is necessary to study ce ll delivery, differentiation and gene… More

DESCRIPTION (provided by applicant): This proposal outlines the development of a new product, and a new methodological approach to the simultaneous measurement of subdermal PO2 and hemoglobin oxygen saturation in skin. The product, Oxyspheres , will find wide use in the development of healing… More