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PROTEIN PURIFICATION USING AN AFFINITY MEMBRANE

Award Information

Agency:
National Science Foundation
Branch:
N/A
Award ID:
10724
Program Year/Program:
1992 / SBIR
Agency Tracking Number:
10724
Solicitation Year:
N/A
Solicitation Topic Code:
N/A
Solicitation Number:
N/A
Small Business Information
BEND RESEARCH, INC.
64550 RESEARCH RD BEND, OR 97701
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Woman-Owned: No
Minority-Owned: No
HUBZone-Owned: No
 
Phase 2
Fiscal Year: 1992
Title: PROTEIN PURIFICATION USING AN AFFINITY MEMBRANE
Agency: NSF
Contract: N/A
Award Amount: $249,680.00
 

Abstract:

THE PROPOSED WORK IS DIRECTED TOWARD THE DEVELOPMENT OF A NOVEL AND HIGHLY SELECTIVE MEMBRANE FOR THE CONTINUOUS PURIFICATION OF VALUABLE PROTEINS SUCH AS INTERFERON FROM COMPLEX AND DILUTE MIXTURES. PRODUCTION OF PROTEINS REQUIRES THREE MAIN STEPS: (1) FERMENTATION OR CELL CULTURE;(2) ISOLATION; AND (3) PURIFICATION. ALTHOUGH FERMENTATION AND CELL-CULTURE PROCESSES HAVE UNDERGONE MUCH DEVELOPMENT RECENTLY, ISOLATION AND PURIFICATION REMAIN THE WEAKEST PARTS OF THE OVERALL SCHEME. THE GOAL OF THE PHASE I PROGRAM IS TO ESTABLISH THE FEASIBILITY OF EMPLOYING "AFFINITY MEMBRANES" TO PURIFY HYDROPHOBIC PROTEINS. THE AFFINITY MEMBRANE CONSISTS OF A MICROPOROUS MEMBRANE IN WHICH THE PORES HAVE BEEN FILLED WITH AN AFFINITY LIGAND THAT SELECTIVELY BINDS THE DESIRED PROTEIN. TRANSPORT OF PROTEINS ACROSS THE FILLED PORES OCCURS BY DISSOLUTION INTO AND DIFFUSION THROUGH THE PORE. IN PHASE I OF THIS PROJECT, THE AFFINITY MEMBRANE WILL BE DEVELOPED AND USED TOSELECTIVELY EXTRACT ALPHA-CHYMOTRYPSIN FROM A MIXTURE OF TWOPROTEINS. ALPHA-CHYMOTRYPSIN IS AN INEXPENSIVE PROTEIN THATWILL BE SUITABLE FOR USE IN THE PHASE I FEASIBILITY WORK.

Principal Investigator:

Paul Van Eikeren
Principal Investigator
5033824100

Business Contact:

Small Business Information at Submission:

Bend Research Inc.
64550 Research Rd Bend, OR 97701

EIN/Tax ID:
DUNS: N/A
Number of Employees: N/A
Woman-Owned: No
Minority-Owned: No
HUBZone-Owned: No