This proposal is to study the synthetic methods to incorporate high-sensitivity electrochemically active functional groups into amines, amino acids and peptides of biological origin. the purpose of this derivatization is to permit the quantitative analysis of picomole levels of these compounds using… More

This proposal concerns the development of a novel immunoassay system based on the fabrication of highly selective amperometric sensors capable of detecting femtomole quantities of material. phase i of this proposal will involve the synthesis of a number of modified surfaces for the amperometric… More

Childhood lead poisoning is stated to be "the nation's leading childhood environmental disease"and being able to accurately determine toxic lead concentrations from small volumes of children's bloodin an accurate and cost effective manner is a considerable challenge. This research describes a… More

The overall objective of this study is to develop a system that can be used to monitor pharmacokinepharmacological or neurochemical events in near real time. This will be accomplished by coupling micdirectly to capillary electrophoresis (CE). The system is much more versatile than conventional… More

Not Available Vehicle simulation capability will be enhanced by developing comprehensive, real-time subsystem models that will be integrated into their multi-body vehicle dynamics simulation. This simulation technology will be used in both driver and hardware-in-the-loop performance evaluations of… More

DESCRIPTION (provided by applicant): This proposal will develop analytical methodology to identify key metabolic pathways and the metabolizing enzymes of botanicals responsible for drug interactions. Initially we will focus on the main active ingredients of widely used… More

DESCRIPTION: (Applicant's abstract) The objective of the proposed work is fast, sensitive integrated clinical analyzers for the diagnosis of two metabolism disorders, homocysteinuria and phenylketonuria (PKU). The assays are based on microchip capillary electrophoresis with… More

DESCRIPTION: (Applicant's abstract) The objective of the proposed work is fast, sensitive integrated clinical analyzers for the diagnosis of two metabolism disorders, homocysteinuria and phenylketonuria (PKU). The assays are based on microchip capillary electrophoresis with… More

DESCRIPTION (provided by applicant): Research laboratories engaged in the discovery of new drugs must be concerned with more than efficacy. Safety pharmacology testing is mandated by regulatory agencies in the USA and Europe. A recent addition to the tests requested by the United States Food and… More

The overall goal of this proposal is the development of the force-plate actometer as an instrument to provide quantitative measurements of rodent behavior, including locomotor activity, tremor, rotational activity and amphetamine-induced focused stereotypies. Unlike video tracking systems, the… More

DESCRIPTION (provided by applicant): Animal models for human disease are important tools for biomedical and pharmaceutical research. Pigs, like humans, develop hypertension, diabetes and cardiovascular disease and respond to stress by releasing hormones via the HPA axis. We propose to utilize pigs… More

DESCRIPTION (provided by applicant): The proposed project will support 'translational medicine', defined as a basic laboratory discovery that becomes applicable to the diagnosis, treatment or prevention of a specific disease . Our goal is to improve the p robability of a laboratory discovery that… More