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AN ACOUSTIC PLATE MODE DNA BIOSENSOR

Award Information

Agency:
Department of Energy
Branch:
N/A
Award ID:
17495
Program Year/Program:
1992 / SBIR
Agency Tracking Number:
17495
Solicitation Year:
N/A
Solicitation Topic Code:
N/A
Solicitation Number:
N/A
Small Business Information
BIODE, INC.
2 OAKWOOD RD CAPE ELIZABETH, ME 04107
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Woman-Owned: No
Minority-Owned: No
HUBZone-Owned: No
 
Phase 1
Fiscal Year: 1992
Title: AN ACOUSTIC PLATE MODE DNA BIOSENSOR
Agency: DOE
Contract: N/A
Award Amount: $50,000.00
 

Abstract:

THIS PROJECT IS DEVELOPING A NEW SOLID-STATE BIOSENSOR TECHNOLOGY FOR BIOLOGICAL MEASUREMENTS. THIS TECHNOLOGY IS BASED ON THE MERGING OF TWO RELATIVELY DIFFERENT TECHNOLOGIES: NUCLEIC ACID PROBES (DNA HYBRIDIZATION TECHNOLOGY) WITH ACOUSTIC PLATE MODE (APM) MICROSENSORS. RECENT ADVANCES IN THE DESIGN AND OPERATION OF APM DEVICES HAVE DEMONSTRATED A DNA HYBRIDIZATION SENSOR PRINCIPLE WITH EXCELLENT SENSITIVITY (NANOGRAMS/MILLILITER), SELECTIVITY, AND TEMPERATURE STABILITY WHEN USED WITH A MODEL PROBE SYSTEM. THIS PROJECT IS THE FIRST ATTEMPT AT THE DIRECT ELECTRONIC IN-SITU SENSING OF A DIAGNOSTICALLY SIGNIFICANT DNA GENE SEQUENCE, ONE THAT CODES FOR THE ABUNDANT LATE MATRIX ANTIGEN OF CYTOMEGALOVIRUS. THIS NEW DNA BIOSENSOR OPERATES IN A CONTINUOUS AND THEREFORE HOMOGENEOUS MODE AND YET IS EXPECTED TO EQUAL OR EXCEED THE SENSITIVITY LIMITS OF EXISTING DOT-BLOT TECHNOLOGIES. THUS, THE BIOSENSOR IS EXPECTED TO RESPOND, IN SITU, OVER TIME TO THE TARGET DNA. IN CONTRAST, THE CURRENT TECHNOLOGY YIELDS A DISCRETE ENDPOINT RESULT. THE BIOSENSOR RESPONSE WILL BE AN ELECTRONIC SIGNAL, ALLOWING NUMEROUS DATA-HANDLING OPTIONS. THE PHASE I PROJECT CONSISTS OF SEVERAL COMPONENTS: (1) AN OPTIMIZED DUAL DELAY LINE APM SENSING ELEMENT IS BEING CONSTRUCTED; (2) A DUAL OSCILLATOR MEASUREMENT SYSTEM FOR THE OPTIMIZED APM IS BEING DESIGNED AND CONSTRUCTED; (3) THE CHEMISTRY EMPLOYED TO COVALENTLY ATTACH THE NUCLEIC ACID PROBE (NAP) IS BEING OPTIMIZED; (4) THE PERFORMANCE OF THE SENSOR IN DETECTING IN-SITU HYBRIDIZATION OF TARGET DNA IS BEING EVALUATED; AND (5) THE CHARACTERIZATION OF THE SENSOR IS TO BE COMPLETED.

Principal Investigator:


0

Business Contact:

Small Business Information at Submission:

Biode Inc
2 Oakwood Dr Cape Elizabeth, ME 04107

EIN/Tax ID:
DUNS: N/A
Number of Employees: N/A
Woman-Owned: No
Minority-Owned: No
HUBZone-Owned: No