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HIGH-SPEED PCR USING A PRESSURIZED GAS THERMOCYCLER

Award Information

Agency:
Department of Health and Human Services
Branch:
N/A
Award ID:
55894
Program Year/Program:
2001 / SBIR
Agency Tracking Number:
2R44GM060113-02
Solicitation Year:
N/A
Solicitation Topic Code:
N/A
Solicitation Number:
N/A
Small Business Information
BIOLINK PARTNERS
109 SCHOOL STREET WATERTOWN, MA 02472-
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Woman-Owned: No
Minority-Owned: No
HUBZone-Owned: No
 
Phase 2
Fiscal Year: 2001
Title: HIGH-SPEED PCR USING A PRESSURIZED GAS THERMOCYCLER
Agency: HHS
Contract: N/A
Award Amount: $508,197.00
 

Abstract:

DESCRIPTION (Applicant's abstract): In Phase I Research, a pressurized gas thermocycler was fabricated and successfully used to amplify gene fragments from human beta-globin (536 b.p.), Human Platelet Antigen (364 b.p.), bacteriophage lambda (333 b.p.), and a bacterial pathogen, E.coli 0157:H7 (91 b.p. and 85 b.p. amplicons). In our fastest experiments, a short 85 b.p. amplicon was amplified through 30 PCR cycles in 78 seconds. The speed and thermal control of this device were limited by its simple 3-valve configuration (hot gas valve, cold gas valve, throttle valve), flow conditioner, inadequate gas cooling, software, and lack of on-line DNA detection optics. In Phase II Research, improved high performance 4- and 5-valve thermocyclers will be fitted with on-line fluorescence detection optics. For short (less than 100 b.p.) amplicons, 30 cycles of amplification/detection will be carried out in less than 60 seconds using pressurized Helium gas. Longer amplicons (100 to 500 b.p.) will be amplified in 1 to 4 minutes using pressurized air. Pressurized gas thermocyclers will enable DNA-based diagnosis of heritable and infectious diseases on an unprecedented time scale. PROPOSED COMMERCIAL APPLICATION: The proposed method is ten times faster than the fastest commercially available hot-air PCR thermocyclers; and 100 times faster than commonly used heat block thermocyclers. Phase II Research will develop enabling technology for high-throughput-screening capabilities, which can be applied to diagnostics, drug discovery, and functional genomics related to heritable and infectious diseases. There is very large and growing market for high-speed PCR for medical/veterinary diagnostics, agribusiness, and biomedical research.

Principal Investigator:

Alex G. Bonner

Business Contact:


6179265450
BIOLINK@MSN.COM
Small Business Information at Submission:

BIOLINK PARTNERS
109 SCHOOL ST WATERTOWN, MA 02172

EIN/Tax ID: 517504137
DUNS: N/A
Number of Employees: N/A
Woman-Owned: No
Minority-Owned: No
HUBZone-Owned: No