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Ultrafiltration Device for Rapid, High, Through-put Sample Preparation

Award Information

Agency:
Department of Health and Human Services
Branch:
N/A
Award ID:
85690
Program Year/Program:
2007 / SBIR
Agency Tracking Number:
GM079876
Solicitation Year:
N/A
Solicitation Topic Code:
N/A
Solicitation Number:
N/A
Small Business Information
BIOLINK PARTNERS
109 SCHOOL STREET WATERTOWN, MA 02472-
View profile »
Woman-Owned: No
Minority-Owned: No
HUBZone-Owned: No
 
Phase 1
Fiscal Year: 2007
Title: Ultrafiltration Device for Rapid, High, Through-put Sample Preparation
Agency: HHS
Contract: 1R43GM079876-01A2
Award Amount: $149,849.00
 

Abstract:

DESCRIPTION (provided by applicant): The goal of this project is to develop advanced ultrafiltration (UF) devices for sample preparation that are faster and easier to use than existing devices. New technology based on proprietary concepts enables ultrafilt ration to be practiced at low pressure, and without the need of centrifuges or other complex apparatus, resulting in a single-pass, tangential-flow ultrafiltration method. The specific aims of the project are: 1. Design and construct prototypes of sing le sample tangential flow, hollow fiber, ultrafiltration devices. 2. Characterize the operating parameters in terms of the processing time and concentration factor as function of fiber geometry, device configuration and vacuum levels for protein solutions, using BSA or mixtures of BSA and peptides as a model. 3. Evaluate the performance of this device on an ultrafiltration application in life science research and compare this performance with published data on the performance of conventional devices. Existing UF devices have inherent limitations which result in slow filtration, high variability, and require the use of centrifuges which is cumbersome and not compatible with the robotic sample handling systems used in many laboratories and necessary fo r high-throughput screening. The UF filtration technology developed in this project combines novel fluidic and geometrical configurations that provide higher throughput and compatibility with robotic systems. The technology developed as a result of the pro posed project is expected to remove barriers in current methods, facilitate the adoption of new diagnostic procedures, and reduce the cost of existing methods. This research will lead to the development of improved technology and new, lower cost devices fo r the purification and isolation of biological samples. Potential applications of the technology developed in this project include disposable devices for single and multiple sample processing, as well as for large-scale systems for biopharmaceutical manufa cturing. The proposed research is expected to produce technology and devices which will provide significant benefits to public health. The research is particularly relevant to improved capabilities for the monitoring of cardiovascular disease states, and b iomarkers in general, and the NHLBI's national efforts to prevent, detect and control hypertension. The technology from this project is expected to improve clinical diagnostic methods involving physiological fluids such as blood and urine, which need to be processed quickly in advance of a clinical testing protocol. The proposed research is expected to produce technology for significant benefits to public health. The research is particularly relevant to improved capabilities for the monitoring of cardio vascular disease states relative to national efforts to prevent, detect and control hypertension. The technology from this project is expected to improve clinical diagnostic methods involving physiological fluids such as blood and urine, which need to be p rocessed quickly in advance of a clinical testing protocol.

Principal Investigator:

Alex G. Bonner
6179265450
BIOLINK@VERIZON.NET

Business Contact:

Alex G. Bonner
biolink@verizon.net
Small Business Information at Submission:

BIOLINK PARTNERS
109 SCHOOL STREET WATERTOWN, MA 02472

EIN/Tax ID: 517504137
DUNS: N/A
Number of Employees: N/A
Woman-Owned: No
Minority-Owned: No
HUBZone-Owned: No