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THE OBJECTIVE OF THIS PROJECT IS TO ESTABLISH INNOVATIVE SCREENING SYSTEMS THAT…

Award Information

Agency:
Department of Health and Human Services
Branch:
N/A
Award ID:
3016
Program Year/Program:
1985 / SBIR
Agency Tracking Number:
3016
Solicitation Year:
N/A
Solicitation Topic Code:
N/A
Solicitation Number:
N/A
Small Business Information
Biological Research Faculty &
10075-20 Tyler Pl Ijamsville, MD 21754
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Woman-Owned: No
Minority-Owned: Yes
HUBZone-Owned: No
 
Phase 1
Fiscal Year: 1985
Title: THE OBJECTIVE OF THIS PROJECT IS TO ESTABLISH INNOVATIVE SCREENING SYSTEMS THAT IDENTIFY NEW CHEMOPREVENTIVE AGENTS, USING DIFFERENT IN VITRO MODELS OF ONCOGENESIS.
Agency: HHS
Contract: N/A
Award Amount: $49,994.00
 

Abstract:

THE OBJECTIVE OF THIS PROJECT IS TO ESTABLISH INNOVATIVE SCREENING SYSTEMS THAT IDENTIFY NEW CHEMOPREVENTIVE AGENTS, USING DIFFERENT IN VITRO MODELS OF ONCOGENESIS. THREE POTENTIAL SYSTEMS, EACH REPRESENTING A DIFFERENT STAGE IN THE CARCINOGENIC PROCESS, WILL BE TESTED AGAINST SELECTED CHEMOPREVENTIVE AGENTS. PHASE I EFFORTS INVOLVE DETERMINATION OF THE INHIBITORY EFFECTS OF RETINOIDS ON: A.IMMORTALIZATION AND MALIGNANT TRANSFORMATION INDUCED BY CARCINOGENIC AGENTS (AFLATOXIN B1, ULTRAVIOLET RADIATION, ONCOGENES) IN VITRO IN NORMAL DIPLOID EPITHELIAL CELL CULTURES DERIVED FROM RAT LIVER. B.EXPRESSION OF THE MALIGNANT PHENOTYPE IN A TEMPERATURE-SENSITIVE MUTANT LINE DERIVED FROM A CHEMICALLY TRANSFORMED LIVER CELL LINE, AND IN THE WILD TYPE CELLS. C.INDUCTION OF MORPHOLOGICAL TRANSFORMATION IN FIBROBLASTIC CELL LINES (NIH 3T3 AND C3H 10T1/2 CLONE 8) BY TRANSFECTION DURING PHASE I, EXPERIMENTAL CONDITIONS AND VARIABLES WILL BE STANDARDIZED FOR EACH OF THE ABOVE MODEL SYSTEMS THE RESULTS OF WHICH ARE EXPECTED TO IDENTIFY THEIR FEASIBILITY AS POTENTIAL SCREENING SYSTEMS FOR CHEMOPREVENTIVE AGENTS. DURING PHASE II, THE APPROPRIATE SYSTEM(S) WILL BE FURTHER DEVELOPED USING DIVERGENT CHEMOPREVENTIVE AGENTS, PRIOR TO THE EVENTUAL COMMERCIALIZATION OF THIS IN VITRO BIOASSAY FOR NEW CHEMOPREVENTIVE AGENTS.

Principal Investigator:

P. thomas iype
PRINCIPAL INVESTIGATOR
3018318810

Business Contact:

Small Business Information at Submission:

Biological Research Faculty &
1007520 Tyler Place Ijamsville, MD 21754

EIN/Tax ID:
DUNS: N/A
Number of Employees: N/A
Woman-Owned: No
Minority-Owned: No
HUBZone-Owned: No