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THE LONG-TERM OBJECTIVE OF THIS PROJECT IS TO ESTABLISH NOVEL RECIPIENT…

Award Information

Agency:
Department of Health and Human Services
Branch:
N/A
Award ID:
4908
Program Year/Program:
1986 / SBIR
Agency Tracking Number:
4908
Solicitation Year:
N/A
Solicitation Topic Code:
N/A
Solicitation Number:
N/A
Small Business Information
Biological Research Faculty &
10075-20 Tyler Pl Ijamsville, MD 21754
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Woman-Owned: No
Minority-Owned: Yes
HUBZone-Owned: No
 
Phase 1
Fiscal Year: 1986
Title: THE LONG-TERM OBJECTIVE OF THIS PROJECT IS TO ESTABLISH NOVEL RECIPIENT EPITHELIAL CELL SYSTEMS THAT WILL DETECT A WIDE SPECTRUM OF ONCOGENES THROUGH DNA TRANSFECTION.
Agency: HHS
Contract: N/A
Award Amount: $50,000.00
 

Abstract:

THE LONG-TERM OBJECTIVE OF THIS PROJECT IS TO ESTABLISH NOVEL RECIPIENT EPITHELIAL CELL SYSTEMS THAT WILL DETECT A WIDE SPECTRUM OF ONCOGENES THROUGH DNA TRANSFECTION. THREE TYPES OF LIVER CELLS REPRESENTING DIFFERENT STAGES OF THE CARCINOGENIC PROCESS WILL BE USED: A NORMAL DIPLOID, AN IMMORTALIZED ANEUPLOID, AND A MUTANT CELL LINE THAT IS TEMPERATURE SENSITIVE FOR TRANSFORMED PHENOTYPE. SPECIFIC AIMS FOR PHASE I INVOLVE: (1) DETERMINATION OF THE TIME COURSE AND DOSE RESPONSE OF DNA UPTAKE IN ORDER TO STANDARD-IZE THE OPTIMAL CONDITIONS OF UPTAKE. THIS WILL BE ACHIEVEDUSING A SENSITIVE ELISA TECHNIQUE THAT ASSAYS FOR BUDR- LABELLED DNA. (2) DETERMINATION OF TRANSFORMATION FREQUEN- CIES INDUCED BY PLASMID DNAS CONTAINING HUMAN HRAS OR V-MYC GENES. CONCLUSIVE RESULTS ON THE BEST TRANSFECTION CONDITIONS (ENHANCEMENT OF DNA UPTAKE BY CELL SURFACE MANIPULATIONS, AND THE EXPRESSION OF ONCOGENE-MEDIATED PHENOTYPE), WILL BE STANDARDIZED. PHASE I RESULTS WILL PROVIDE A STANDARDIZED PROTOCOL FOR FUTURE DNA TRANSFECTION EXPERIMENTS; THESE ARE PLANNED FOR PHASE II USING OTHER CELLTYPES AND ONCOGENES.

Principal Investigator:

P thomas iype
PRINCIPAL INVESTIGATOR
3018318810

Business Contact:

Small Business Information at Submission:

Biological Research Faculty &
10075-20 Tyler Place Ijamsville, MD 21754

EIN/Tax ID:
DUNS: N/A
Number of Employees: N/A
Woman-Owned: No
Minority-Owned: No
HUBZone-Owned: No