ULTRASENSITIVE DETECTION SYSTEM FOR LOW-LEVEL TOXICITY

Award Information

Agency: Department of Health and Human Services	Branch: N/A	Award ID: 13852	Program Year/Program: 1993 / SBIR
Agency Tracking Number: 13852	Solicitation Year: N/A	Solicitation Topic Code: N/A	Solicitation Number: N/A

Small Business Information: Biological Research Faculty &
10075-20 Tyler Pl Ijamsville, MD 21754
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Woman-Owned: No

Minority-Owned: Yes

HUBZone-Owned: No

Phase 2: Fiscal Year: 1993

Title: ULTRASENSITIVE DETECTION SYSTEM FOR LOW-LEVEL TOXICITY

Agency: HHS

Contract: N/A

Award Amount: $411,484.00

Abstract:
THE LONG-TERM OBJECTIVE OF THIS PROJECT IS TO DEVELOP A LABORATORY TEST FOR MONITORING LOW-LEVEL TOXICITY FROM CHEMICAL EXPOSURE AT LEVELS THAT DO NOT PRODUCE ACUTE SYMPTOMS SUCH AS CYTOTOXICITY, BUT PRODUCE DETECTABLE DAMAGE LATER. THE SPECIFIC AIMS FOR THE PHASE I INVESTIGATIONS ARE TO: (1) DETERMINE THE SUBCYTOTOXIC LEVELS OF THREE ENVIRONMENTALAGENTS-A MYCOTOXIN, A POLYCYCLIC HYDROCARBON, AND AN AROMATIC AMINE; (2) SET UP A FAIRLY LONG-TERM (3 TO 4 MONTHS) BIOASSAY USING A WELL-CHARACTERIZED RAT LIVER EPITHELIAL CELL LINE, EXPOSED CHRONICALLY TO THE ABOVE SUBCYTOTOXIC DOSES OF CARCINOGENS; AND (3) MONITOR THE EMERGENCE OF A POPULATION OF CELLS CONTAINING MUTATED RAS ONCOGENE USING MOLECULAR BIOLOGICAL ENDPOINTS. THE TECHNICAL INNOVATIONS IN THESE STUDIES INCLUDE POLYMERASE CHAIN REACTION AMPLIFICATION OF LOW LEVELS OF CHEMICALLY INDUCED MUTATIONS IN AN ONCOGENE FROM MAMMALIAN CELLS AND THEIR MEASUREMENT AND DETECTION WITH A LIQUID HYBRIDIZATION/GEL RETARDATION ASSAY.

Principal Investigator:
Narayan M Shivapurkar
3018318810

Business Contact:

Small Business Information at Submission:

Biological Research Faculty &
10075-20 Tyler Pl Ijamsville, MD 21754

EIN/Tax ID:

DUNS: N/A

Number of Employees: N/A

Woman-Owned: No

Minority-Owned: No

HUBZone-Owned: No