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DIFFERENTIATED CELLS AS TARGETS FOR CARCINOGEN-SCREENING

Award Information

Agency:
Department of Health and Human Services
Branch:
N/A
Award ID:
19221
Program Year/Program:
1992 / SBIR
Agency Tracking Number:
19221
Solicitation Year:
N/A
Solicitation Topic Code:
N/A
Solicitation Number:
N/A
Small Business Information
Biological Research Faculty &
10075-20 Tyler Pl Ijamsville, MD 21754
View profile »
Woman-Owned: No
Minority-Owned: Yes
HUBZone-Owned: No
 
Phase 1
Fiscal Year: 1992
Title: DIFFERENTIATED CELLS AS TARGETS FOR CARCINOGEN-SCREENING
Agency: HHS
Contract: N/A
Award Amount: $50,000.00
 

Abstract:

THE OVERALL OBJECTIVE IS TO DEVELOP A NEW SYRIAN HAMSTER EMBRYO (SHE) MORPHOLOGICAL TRANSFORMATION ASSAY (MTA) EMPLOYING SPECIFIC CELL TYPES AS TARGETS AND USING DEFINED, SERUM-FREE CULTURE MEDIA. SPECIFIC AIMS FOR PHASE I STUDY INCLUDE: (1) ISOLATION AND CHARACTERIZATION OF SPECIFIC CELL TYPES (CHONDROCYTES, MYCOCYTES, FIBROBLASTS, ENDOTHELIAL CELLS) FROM SHE AS TARGETS FOR THE IN VITRO TRANSFORMATION ASSAY; (2) ADAPTATION OF EACH OF THE SELECTED TARGET CELL TYPES TO GROW IN APPROPRIATE SERUM-FREE GROWTH MEDIA; (3) DEVELOPMENT OF A PROTOTYPE SERUM-FREE MEDIUM THAT ENHANCES THE TRANSFORMED PHENOTYPE. TECHNICAL INNOVATION IN THIS PROJECT LIES IN THE SUBSTITUTION OF DEFINED FACTORS IN PLACE OF SERUM AND AVOIDANCE OF THE USE OF X-IRRADIATED "FEEDER" CELLS THAT MIGHT REPRESENT PREFERRED TARGETS FOR PARTICULAR CARCINOGENS WOULD CONSTITUTE AN IMPORTANT IMPROVEMENT OVER CURRENT MTAS. PROCEDURES AND REAGENTS DEVELOPED WILL BE USEFUL IN SCREENING ENVIRONMENTAL CHEMICALS FOR THEIR CARCINOGENIC POTENTIAL, HENCE COMMERCIALLY VALUABLE.

Principal Investigator:

P Thomas Iype
3018318810

Business Contact:

Small Business Information at Submission:

Biological Research
10075-20 Tyler Place Ijamsville, MD 21754

EIN/Tax ID:
DUNS: N/A
Number of Employees: N/A
Woman-Owned: No
Minority-Owned: No
HUBZone-Owned: No