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DEVELOPMENT OF A DNA PROBE FOR THE RAPID DETECTION OF OF MYCOMPLASMA…

Award Information

Agency:
Department of Agriculture
Branch:
N/A
Award ID:
1900
Program Year/Program:
1986 / SBIR
Agency Tracking Number:
1900
Solicitation Year:
N/A
Solicitation Topic Code:
N/A
Solicitation Number:
N/A
Small Business Information
Bionique Laboratorytories I
Bloomingdale Road Saranac Lake, NY 12983
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Woman-Owned: No
Minority-Owned: No
HUBZone-Owned: No
 
Phase 2
Fiscal Year: 1986
Title: DEVELOPMENT OF A DNA PROBE FOR THE RAPID DETECTION OF OF MYCOMPLASMA GALLISEPTICUM
Agency: USDA
Contract: N/A
Award Amount: $150,198.00
 

Abstract:

THE OBJECTIVE OF THIS PROJECT IS TO CONSTRUCT A MYCOMPLASMA GALLISEPTICUM BIOTINYLATED DNA PROBE. ECONOMIC LOSSES FROM M. GALLISEPTICUM INFECTION ARE ESTIMATED TO BE $118 MILLION ANNUALLY IN THE UNITED STATES. MINIMUM EFFICIENCY OF M. GALLISEPTICUM DISEASE CONTROL REQUIRES A RAPID AND SENSITIVEIDENTIFICATION SYSTEM SUCH AS A PROBE. MYCOPLASMA GAL GALLISEPTICUM DNA WILL BE LABELED WILL BIOTIN-11-DUTP IN A STANDARD NICK-TRANSLATION REACTION. TARGET DNA HYBRIDIZED WITH THE BIOTINYLATED PROBE ON A NITROCELLULOSE FILTER WILL BE INCUBATED WITH STREPTAVIDIN AND POLYALKALINE PHOSPHASTASE. UPON ADDITION OF AN ENZYME/SUBSTRATE COMPLEX, THE HYBRIDIZED DNA WILL APPEAR AS A BLUE SPOT. RESULTS CAN BE OBTAINED IN 24-36 HOURS, WHICH IS CONSIDERABLY FASTER THAN ROUTINE METHODS CURRENTLY IN USE.

Principal Investigator:


0

Business Contact:

Steven j. geary
PRINCIPAL INVESTIGATOR
5188912356
Small Business Information at Submission:

Bionique Laboratorytories I
Bloomingdale Road Saranac Lake, NY 12983

EIN/Tax ID:
DUNS: N/A
Number of Employees: N/A
Woman-Owned: No
Minority-Owned: No
HUBZone-Owned: No