DESCRIPTION (provided by applicant): The overall goal of this proposal is to develop a new cost-effective method for the production of high-purity polyclonal antibodies. Polyclonal antibodies are important reagents used worldwide for antigen detection and purification in basic research as well as… More

This Small Business Innovation Research (SBIR) Phase I research project aims to develop an improved method for the delivery of small inhibitory ribonucleic acids (siRNA) into cells. The proposed methodology will utilize chemically induced immuno-conjugates or direct linking of siRNAs to antibodies… More

It is important to test the food supply for known toxic or banned substances which can impinge public safety. While a number of high-throughput tests have been developed to screen the food supply for higher molecular weight banned substances, it has been difficult to develop methods to… More

DESCRIPTION (provided by applicant): The overall goal of this proposal is to develop novel reagents to efficiently deliver functional siRNA into animals for gene silencing experiments. Pharmaceutical and biotechnology companies are actively testing siRNAs as therapeutic agents and to validate drug… More

DESCRIPTION (provided by applicant): One of the major underlying factors for the significant broad impact of molecular biology on biomedical research is the generation of effective and highly reproducible assay kits to measure fundamental properties of ge ne expression. One area of molecular… More

The research will result in new kits for quantitative detection and monitoring of hypoxia inducible factor-1 alpha (Hif-1a), a promising cellular target protein for pharmacological intervention. During the Phase 1 project, monoclonal antibodies will be dev eloped and used to create highly sensitive… More

This award is funded under the American Recovery and Reinvestment Act of 2009 (Public Law 111-5). This Small Business Innovation Research (SBIR) Phase II project will develop technologies that optimize the use of RNA interference (RNAi) in animals. RNAi is an invaluable tool for characterizing gene… More

The safety of the public food supply is an emerging and important public health issue in the United States. Recent, widely-reported food and feed contaminations by pathogens and banned substances such as melamine have fueled an increased demand for improved assays to screen and protect the food… More

Protecting the integrity of the nation's food from contamination by toxins is a very serious challenge for our country. Melamine, in particular, is a toxic substance which has recently emerged as a serious threat to the safety of the U.S. food supply. Recent, widely-reported food and feed… More

This Small Business Innovation Research (SBIR) Phase I project proposes to examine high throughput methods to quantify intacellular microRNA (miRNA) concentrations in cells that have shown to be associated with normal physiological processes as well as diseases including cancer. Currently, there are… More

DESCRIPTION (provided by applicant): While changes in mRNA decay rates account for approximately 50% of the regulation of gene expression in the cell, an assay to accurately and reliably measure mRNA half lives is currently not available. In particular, the deficiency of reliable commercial kits… More

DESCRIPTION (provided by applicant): Changes in expression have been shown to be associated with a variety of normal physiological processes as well as diseases including cancer. Studies have already shown that miRNAs may provide useful markers for the development of disease diagnostic and… More

DESCRIPTION (provided by applicant): Oil spills can cause tremendous environmental damage and are a serious threat to public health. Petroleum contains many toxic compounds such as polyaromatic hydrocarbons (PAHs). Some PAH compounds are highly carcinogenic and cause DNA mutations in humans. When… More

This Small Business Innovation Research Phase II project examines high throughput methods to quantify intacellular microRNA (miRNA) concentrations in cells that have shown to be associated with normal physiological processes, as well as diseases, including cancer. Currently there are no rapid,… More

Flaviviruses are a major health threat worldwide. This family of RNA viruses causes a variety of deadly diseases such as Dengue Fever, Yellow Fever, West Nile virus, and Tick-borne Encephalitis. There are currently no effective means to control these viruses. New drugs to counteract these viruses… More

This Small Business Innovation Research (SBIR) Phase I project aims to make next generation sequencing technology for small RNA more quantitative and less biased. High throughput sequencing has transformed the landscape of genomic research with its ability to produce gigabases of data in a single… More