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DNA Amplification from Microdissected Chromosomes

Award Information

Agency:
Department of Health and Human Services
Branch:
N/A
Award ID:
25103
Program Year/Program:
1996 / SBIR
Agency Tracking Number:
25103
Solicitation Year:
N/A
Solicitation Topic Code:
N/A
Solicitation Number:
N/A
Small Business Information
Genaissance Pharmaceuticals
Five Science Park New Haven, CT 06511
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Woman-Owned: No
Minority-Owned: No
HUBZone-Owned: No
 
Phase 2
Fiscal Year: 1996
Title: DNA Amplification from Microdissected Chromosomes
Agency: HHS
Contract: 1 R43 GM50658-1,
Award Amount: $750,000.00
 

Abstract:

We will develop kits, services, and reagents useful in the processes of generating and employingamplified DNA mini-libraries (i.e., amplified products) from microdissected human chromosomefragments. The focus of Phase I studies will be development of standard protocols for generating suchmini-libraries. Two amplification strategies are proposed based on their use of recognition sitesdistributed throughout the human genome at about 250 bp intervals (insuring representative coverageof genomic sequences in the min-library). The strategies are based on DNA sequence recognition byMbo I cleavage and by oligonucleotide hybridization to the most common 6 bp sequences in the humangenome. DNA products obtained from both strategies are PCR amplified with a single adaptoroligonucleotide. The products of the amplification reactions will be analyzed for the amount and sizeof DNA, degree of genome coverage, hybridization specificity, retrievability upon reamplification, easeof use and reproducibility. Based on amplification strategies developed in Phase I, we will ultimatelymake available to the human genom research community standardized reagents that can be used: a) forobtaining region-specific clones (i.e., genomic clones for contig assembly/map closure, and/or cDNAclones to identify novel genes), b) as starting material for isolation of region-specific STSs, and c) ashybridization/amplification targets for subchromosome localization.

Principal Investigator:

Clark Huckaby
2037731450

Business Contact:

Small Business Information at Submission:

Bios Laboratorytories, Inc.
5 Science Park New Haven, CT 06511

EIN/Tax ID:
DUNS: N/A
Number of Employees: N/A
Woman-Owned: No
Minority-Owned: No
HUBZone-Owned: No