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Directed Multiplex DNA Sequencing by Hybridization

Award Information

Agency:
Department of Energy
Branch:
N/A
Award ID:
34690
Program Year/Program:
1997 / SBIR
Agency Tracking Number:
34690
Solicitation Year:
N/A
Solicitation Topic Code:
N/A
Solicitation Number:
N/A
Small Business Information
Genaissance Pharmaceuticals
Five Science Park New Haven, CT 06511
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Woman-Owned: No
Minority-Owned: No
HUBZone-Owned: No
 
Phase 2
Fiscal Year: 1997
Title: Directed Multiplex DNA Sequencing by Hybridization
Agency: DOE
Contract: DE-FG02-96ER82136
Award Amount: $750,000.00
 

Abstract:

41186 BIOS Laboratories, Inc. The ultimate goal of the Human Genome program is to obtain the entire sequence of the genetic makeup of the human body. This research has mostly encompassed large scale DNA sequencing projects, with interdisciplinary teams of biochemists, engineers, and computer specialists, which are beyond the resources of small to mid-sized laboratories. This project will develop an alternate, lower cost technique for directed DNA sequencing, utilizing hybridizations of Peptide Nucleic Acid (PNA) oligomer probes. PNA oligomers hybridize more stably and specifically to DNA targets than conventional oligonucleotides. Phase I is concerned with improvements in the three multiply repeated steps in the directed sequencing process: (1) probe assembly, (2) sequencing reactions and (3) gel electrophoresis. The technique allows the efficiency of directed sequencing, while avoiding most of the bottlenecks identified for the process. Commercial Applications and Other Benefits as described by the awardee: The development of modular PNA probes from pre-synthesized libraries should substantially reduce the cost of PNAs and extend further the applications of these novel molecules to DNA sequencing. The resources to be developed include PNA oligomer libraries, anchor primer kits for multiplex sequencing, and customized services.

Principal Investigator:

Dr. Gualberto Ruano
Scientific Director
2037731450

Business Contact:

Mr. Kevin Rakin
Chief Financial Officer
2037731450
Small Business Information at Submission:

Genaissance Pharmaceuticals
Five Science Park New Haven, CT 06511

EIN/Tax ID:
DUNS: N/A
Number of Employees: N/A
Woman-Owned: No
Minority-Owned: No
HUBZone-Owned: No