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DEVELOPMENT OF AN ANTIGEN CAPTURE ASSAY FOR HTLV-I

Award Information

Agency:
Department of Health and Human Services
Branch:
N/A
Award ID:
11101
Program Year/Program:
1989 / SBIR
Agency Tracking Number:
11101
Solicitation Year:
N/A
Solicitation Topic Code:
N/A
Solicitation Number:
N/A
Small Business Information
SERACARE LIFE SCIENCES, INC.
SERACARE LIFE SCIENCES, INC. 1935 AVENIDA DEL ORO, STE F OCEANSIDE, MD 92056 0582
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Woman-Owned: No
Minority-Owned: No
HUBZone-Owned: No
 
Phase 1
Fiscal Year: 1989
Title: DEVELOPMENT OF AN ANTIGEN CAPTURE ASSAY FOR HTLV-I
Agency: HHS
Contract: N/A
Award Amount: $49,891.00
 

Abstract:

PHASE I RESEARCH IILL DEVELOP A PROTOTYPE ELISA ANTIGEN-CAPTURE KIT TO MEASURE HTLV-I CORE PROTEINS P19 AND P24 PRODUCED DURING VIRUS REPLICATION IN CELLS. HTLV-I IS LINKED TO THE DEVELOPMENT OF ADULT T-CELL LEUKEMIA AND TOTROPICAL SPASTIC PARAPARESIS, A NEUROLOGICAL DISEASE OF INTEREST TO RESEARCHERS ON MULTIPLE SCLEROSIS. TRACKING THE PROGRESS OF HTLV-I INFECTION IN TISSUE CULTURES IS MORE DIFFICULT THAN TRACKING HIV INFECTION: HTLV-I SELDOM KILLS THE CELLS IT INFECTS, AND THE REVERSE TRANSCRIPTASE (RT) IT PRODUCES IS NOT VERY ACTIVE IN CURRENT RT ASSAYS. THEREFORE, A COMMERCIAL KIT THAT MEASURES THE CORE PROTEINS PRODUCED DURING INFECTION WILL BEVALUABLE IN STUDIES OF HTLV-I IN TISSUE CULTURE. THE PROTOTYPE ASSAY WILL USE P19 AND P24 ANTIBODIES AS CAPTURE ANTIBODIES. HIGH-TITER HUMAN SERA WILL BE USED AS THE DETECTOR ANTIBODY UNTIL HYPERIMMUNE RABBIT SERA IS AVAILABLE. THE PROTOTYPE ASSAY WILL BE MORE SENSITIVE THAN RT ASSAYS IN TRACKING HTLV-I INFECTION, BUT ITS SENSITIVITY WILL BE LIMITED BY THE CURRENT LACK OF VERY HIGH-AFFINITY MONOCLONAL ANTIBODIES FOR P19 AND P24. PRODUCTION OF HIGH-AFFINITY MONOCLONAL ANTIBODIES WILL BEGIN IN PHASE I.

Principal Investigator:


0

Business Contact:

Small Business Information at Submission:

Biotech Research Laboratories
1600 E Gude Dr Rockville, MD 20850

EIN/Tax ID:
DUNS: N/A
Number of Employees: N/A
Woman-Owned: No
Minority-Owned: No
HUBZone-Owned: No