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AN ON-LINE MICROBIOLOGICAL ANALYZER

Award Information

Agency:
National Aeronautics and Space Administration 		Branch:
N/A 		Award ID:
17100 		Program Year/Program:
1992 / SBIR
Agency Tracking Number:
17100 		Solicitation Year:
N/A 		Solicitation Topic Code:
N/A 		Solicitation Number:
N/A
Small Business Information
Biotronics Technologies, Inc.
W226 N555B Eastmound Drive Waukesha, WI 53186
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Woman-Owned: No
Minority-Owned: No
HUBZone-Owned: No
 
Phase 2
Fiscal Year: 1992
Title: AN ON-LINE MICROBIOLOGICAL ANALYZER
Agency: NASA
Contract: N/A
Award Amount: $499,707.00
 

Abstract:

MICROBIOLOGICAL MONITORING WILL BE A CRITICAL FUNCTION IN THE BIOMASS-PROCESSING REACTORS OF THE NASA CONTROLLED ECOLOGICAL LIFE SUPPORT SYSTEM (CELSS). MEASUREMENT OF CELLDENSITY AND CELL ACTIVITY IN SOME REACTORS WILL ALLOW FOR THE AUTOMATION AND OPTIMIZATION OF BIOCHEMICAL PROCESSES WITHOUT THE NEED FOR EXPERIENCED SKILLED PERSONNEL. IN OTHER REACTORS, MICROBIOLOGICAL DETECTION AND CHARACTERIZATION OF BACTERIAL CONTAMINANTS WILL BE REQUIRED. ALL LIVING CELLS, INCLUDING MICRO-ORGANISMS, CONTAIN NICOTINAMIDE ADENINE DINUCLEOTIDES (NAD) THAT SERVE AS COFACTORS IN MANY METABOLIC REACTIONS. THE REDUCED FORMS(NAD(P)H) ARE HIGH-ENERGY MOLECULES THAT FLUORESCE AT 460 NM WHEN IRRADIATED WITH LIGHT AT 340 NM. THIS FLUORESCENCE PROPERTY OF NADPH MAY BE USED TO MEASURE MICROORGANISM CELL CONCENTRATION AND ACTIVITY USING A FIBER-OPTIC FLUORESCENCE ANALYZER, SUCH AS THE BIOTRONICS BI-401. THE BI-401 IS AN ON-LINE, FIBER-LINKED, MULTIPLE-WAVELENGTH FLUOROMETRIC ANALYZER THAT PROVIDES AN IDEAL EXPERIMENTAL VEHICLE FOR RESEARCH IN BIOCHEMICAL-PROCESS ANALYSIS AND CONTROL. THE MULTIPLE-WAVELENGTH NATURE OF THE BI-401 WILL ALLOW FOR INVESTIGATION OF NADPH AND OTHER NATURAL FLUOROPHORES SUCH AS TRYPTOPHAN, TYROSINE, AND ATP. CELL MASS AND ACTIVITY LEVELS, ALONG WITH CELL METABOLIC STATE, WILL BE THE PRIMARYAREAS OF INVESTIGATION. MICROBIOLOGICAL MONITORING WILL BE A CRITICAL FUNCTION IN THE BIOMASS-PROCESSING REACTORS OF THE NASA CONTROLLED ECOLOGICAL LIFE SUPPORT SYSTEM (CELSS). MEASUREMENT OF CELLDENSITY AND CELL ACTIVITY IN SOME REACTORS WILL ALLOW FOR THE AUTOMATION AND OPTIMIZATION OF BIOCHEMICAL PROCESSES WITHOUT THE NEED FOR EXPERIENCED SKILLED PERSONNEL. IN OTHER REACTORS, MICROBIOLOGICAL DETECTION AND CHARACTERIZATION OF BACTERIAL CONTAMINANTS WILL BE REQUIRED. ALL LIVING CELLS, INCLUDING MICRO-ORGANISMS, CONTAIN NICOTINAMIDE ADENINE DINUCLEOTIDES (NAD) THAT SERVE AS COFACTORS IN MANY METABOLIC REACTIONS. THE REDUCED FORMS(NAD(P)H) ARE HIGH-ENERGY MOLECULES THAT FLUORESCE AT 460 NM WHEN IRRADIATED WITH LIGHT AT 340 NM. THIS FLUORESCENCE PROPERTY OF NADPH MAY BE USED TO MEASURE MICROORGANISM CELL CONCENTRATION AND ACTIVITY USING A FIBER-OPTIC FLUORESCENCE ANALYZER, SUCH AS THE BIOTRONICS BI-401. THE BI-401 IS AN ON-LINE, FIBER-LINKED, MULTIPLE-WAVELENGTH FLUOROMETRIC ANALYZER THAT PROVIDES AN IDEAL EXPERIMENTAL VEHICLE FOR RESEARCH IN BIOCHEMICAL-PROCESS ANALYSIS AND CONTROL. THE MULTIPLE-WAVELENGTH NATURE OF THE BI-401 WILL ALLOW FOR INVESTIGATION OF NADPH AND OTHER NATURAL FLUOROPHORES SUCH AS TRYPTOPHAN, TYROSINE, AND ATP. CELL MASS AND ACTIVITY LEVELS, ALONG WITH CELL METABOLIC STATE, WILL BE THE PRIMARYAREAS OF INVESTIGATION.

Principal Investigator:


0

Business Contact:

Small Business Information at Submission:

Biotronics Technologies Inc.
12020 W Ripley Ave Wauwatosa, WI 53226

EIN/Tax ID:
DUNS: N/A
Number of Employees: N/A
Woman-Owned: No
Minority-Owned: No
HUBZone-Owned: No