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PROGRAMMABLE ULTRAVIOLET ILLUMINATOR USING AN AOTF

Award Information

Agency:
Department of Health and Human Services
Branch:
N/A
Award ID:
16574
Program Year/Program:
1991 / SBIR
Agency Tracking Number:
16574
Solicitation Year:
N/A
Solicitation Topic Code:
N/A
Solicitation Number:
N/A
Small Business Information
Brimrose Corporation of America
19 Loveton Circle Hunt Valley Loveton Center Sparks, MD 21152-9201
View profile »
Woman-Owned: No
Minority-Owned: No
HUBZone-Owned: No
 
Phase 1
Fiscal Year: 1991
Title: PROGRAMMABLE ULTRAVIOLET ILLUMINATOR USING AN AOTF
Agency: HHS
Contract: N/A
Award Amount: $47,210.00
 

Abstract:

INCREASING USE OF FURA-2 AND OTHER DUAL EXCITATION PROBES HAVE HIGHLIGHTED THE ADVANTAGES OF THE RATIO METHOD FOR DETERMINING INTRACELLULAR ION CONCENTRATIONS. THIS HAS FOSTERED THE DEVELOPMENT OF NEW PROBES (E. G., FOR NA+, K+, H+, AND MG2+) WITH WHICH ION CONCENTRATIONS ARE DETERMINED AS A FUNCTION OF THE RATIO OF THE FLUORESCENCE EMISSION EXCITED BY ILLUMINATION AT TWO WAVELENGTHS (DUAL EXCITATION). UNFORTUNATELY, THE DEVELOPMENT OF RELATIVELY INEXPENSIVE ULTRAVIOLET (UV) AND VISIBLE FLUORESCENCE ILLUMINATION SYSTEMS FOR USE WITH THESE PROBES LAGS BEHIND THE DEVELOPMENT OF THE PROBES THEMSELVES. THIS IS ESPECIALLY TRUE WHEN THE PROBES ARE USED FOR DIGITAL IMAGING, WHERE EXCITATORY ILLUMINATION MUST BE GATED SYNCHRONOUSLY WITH THE VIDEO FRAMES. TO ADDRESS THIS PROBLEM, AN OPTIMIZED ACOUSTO-OPTIC TUNABLE FILTER (AOTF) WILL BE FABRICATED AND INCORPORATED INTO A NEW ULTRAVIOLET AND VISIBLE ILLUMINATOR FOR DUAL EXCITATION PROBES. THE ILLUMINATOR WILL BE DEMONSTRATED IN A FLUORESCENCE MICROSCOPY APPLICATION, WHERE THE USE OF AOTF TECHNOLOGY WILL PERMIT FAST AND ACCURATE CONTROL THE WAVELENGTH, TIMING AND INTENSITY OF THE EXCITATION. THE DESIGN OF THE AOTF AND ILLUMINATOR WILL MAXIMIZE THE FILTERED OPTICAL POWER IN THE 330 TO 350 NM RANGE FOR USE WITH FURA-2 AND OTHER DUAL EXCITATION PROBES.

Principal Investigator:

Rosemeier, Ronald G
Principal Investigator
4109317200

Business Contact:

1 r43 es05681-01
Small Business Information at Submission:

Brimrose Corpon Of Americ
5020 Campbell Boulevard Baltimore, MD 21236

EIN/Tax ID:
DUNS: N/A
Number of Employees: N/A
Woman-Owned: No
Minority-Owned: No
HUBZone-Owned: No