USA flag logo/image

An Official Website of the United States Government

THE AIM OF THIS PROJECT IS TO DEVELOP CLONING VECTORS THAT DIRECT THE…

Award Information

Agency:
Department of Health and Human Services
Branch:
N/A
Award ID:
3187
Program Year/Program:
1985 / SBIR
Agency Tracking Number:
3187
Solicitation Year:
N/A
Solicitation Topic Code:
N/A
Solicitation Number:
N/A
Small Business Information
California Biotechnology, Inc.
2450 Bayshore Parkway Mountain View, CA 94043
View profile »
Woman-Owned: No
Minority-Owned: No
HUBZone-Owned: No
 
Phase 1
Fiscal Year: 1985
Title: THE AIM OF THIS PROJECT IS TO DEVELOP CLONING VECTORS THAT DIRECT THE HIGH-LEVEL SYNTHESIS AND SECRETION OF HETEROLOGOUS PROTEIN PRODUCTS IN THE YEAST SACCHAROMYCES CEREVISIAE.
Agency: HHS
Contract: N/A
Award Amount: $50,000.00
 

Abstract:

THE AIM OF THIS PROJECT IS TO DEVELOP CLONING VECTORS THAT DIRECT THE HIGH-LEVEL SYNTHESIS AND SECRETION OF HETEROLOGOUS PROTEIN PRODUCTS IN THE YEAST SACCHAROMYCES CEREVISIAE. A CRITICAL COMPONENT NEEDED FOR SUCH VECTORS IS A DNA SEQUENCE CODING FOR A SECRETION SIGNAL RECOGNIZED BY YEAST. THE GENE FOR THE SECRETED K2 KILLER TOXIN OF S. CEREVISIAE WILL BE ANALYZED TO DETERMINE IF PORTIONS OF THIS GENE CAN BE USED AS THE SECRETION SIGNAL IN THE VECTORS. CDNA CLONES DERIVED FROM THE DOUBLE-STRANDED RNA RESPONSIBLE FOR K2 TOXIN PRODUCTION WILL BE ANALYZED BY SEQUENCING AND BY EXPRESSION IN YEAST CELLS TO IDENTIFY CLONES THAT CODE FOR THE TOXIN PROTEIN. TO DETERMINE WHETHER THE TOXIN SEQUENCES WILL DIRECT THE SECRETION OF HETEROLOGOUS PRODUCTS, VARIOUS FRAGMENTS OF THE TOXIN CDNAS WILL THEN BE FUSED WITH MAMMALIAN GENES, PLACED INTO YEAST EXPRESSION VECTORS, AND TRANSFORMED INTO YEAST CELLS TO ASSAY FOR EXPRESSION AND SECRETION OF THE FOREIGN PROTEINS. IF SECRETION IS DETECTED, THEN A SECOND PHASE OF THE PROJECT WILL BEGIN. THIS PHASE WILL PRIMARILY INVOLVE A MORE DETAILED ANALYSIS OF THE TOXIN GENE, IN ORDER TO OPTIMIZE THE TOXIN ELEMENTS INCORPORATED INTO THE VECTORS.

Principal Investigator:

Judith abraham
PRINCIPAL INVESTIGATOR
4159661550

Business Contact:

Small Business Information at Submission:

California Biotechnology Inc
2450 Bayshore Frontage Rd. Mountain View, CA 94043

EIN/Tax ID:
DUNS: N/A
Number of Employees: N/A
Woman-Owned: No
Minority-Owned: No
HUBZone-Owned: No