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AFFINITY MATRICES FOR PROCESS SCALE PURIFICATION

Award Information

Agency:
Department of Health and Human Services
Branch:
N/A
Award ID:
13876
Program Year/Program:
1990 / SBIR
Agency Tracking Number:
13876
Solicitation Year:
N/A
Solicitation Topic Code:
N/A
Solicitation Number:
N/A
Small Business Information
California Biotechnology, Inc.
2450 Bayshore Parkway Mountain View, CA 94043
View profile »
Woman-Owned: No
Minority-Owned: No
HUBZone-Owned: No
 
Phase 1
Fiscal Year: 1990
Title: AFFINITY MATRICES FOR PROCESS SCALE PURIFICATION
Agency: HHS
Contract: N/A
Award Amount: $49,790.00
 

Abstract:

THE AIM OF THIS RESEARCH IS TO INVESTIGATE THE USE OF NEW AFFINITY MATRICES IN PURIFICATION AND CLEAVAGE OF RECOMBINANT PROTEINS AT PROCESS SCALE. THE PERFORMANCE OF DIFFERENT AFFINITY MATRICES FOR IMMOBILIZATION OF ENZYMESWILL BE DIRECTLY COMPARED. SCALABILITY AND PERFORMANCE WILLBE EVALUATED IN RELATION TO COST. IN PARTICULAR, THE PERFORMANCE OF MEMBRANE-IMMOBILIZED ENZYME WILL BE COMPARED TO SOFT-GEL AND RIGID-BEAD IMMOBILIZED ENZYMES. THE RESULTS COULD AID IN THE DESIGN OF AFFINITY MATRICES USEFUL FOR PROCESS SCALE WORK. BY STREAMLINING PURIFICATIONPROCESSES SIGNIFICANTLY, APPLICABLE AFFINITY METHODS COULD FACILITATE THE DEVELOPMENT OF NEW PROTEIN THERAPEUTICS. A SMALL DOMAIN THAT CAN BE PURIFIED BY A SCALABLE AFFINITY CHROMATOGRAPHY METHOD WILL BE DEFINED BY PEPTIDE SYNTHESIS. PEPTIDE SEQUENCES TAKEN FROM CALCIUM-BINDING DOMAINS WILL BE SYNTHESIZED. AFTER INITIAL SCREENING TO IDENTIFY PEPTIDES HAVING AFFINITY FOR CA(2+), AN AFFINITY PURIFICATION METHOD BASED UPON METAL BINDING WILL BE OPTIMIZED. THE ULTIMATE GOAL IS TO EXPRESS THE SHORTEST ACTIVE SEQUENCE RECOMBINANTLY IN FUSION WITH POTENTIAL PRODUCT PROTEIN SEQUENCES.

Principal Investigator:

Paula J Shadle
4159661550

Business Contact:

Small Business Information at Submission:

California Biotechnology Inc
2450 Bayshore Pkwy Mountain View, CA 94043

EIN/Tax ID:
DUNS: N/A
Number of Employees: N/A
Woman-Owned: No
Minority-Owned: No
HUBZone-Owned: No