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HIGH PERFORMANCE IMMOBILIZED METAL AFFINITY CHROMATOGRAPHY

Award Information

Agency:
National Science Foundation
Branch:
N/A
Award ID:
21647
Program Year/Program:
1996 / SBIR
Agency Tracking Number:
21647
Solicitation Year:
N/A
Solicitation Topic Code:
N/A
Solicitation Number:
N/A
Small Business Information
Chromatochem Inc.
2837 Ft Missoula Rd Missoula, MT 59801
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Woman-Owned: No
Minority-Owned: No
HUBZone-Owned: No
 
Phase 2
Fiscal Year: 1996
Title: HIGH PERFORMANCE IMMOBILIZED METAL AFFINITY CHROMATOGRAPHY
Agency: NSF
Contract: N/A
Award Amount: $10,000.00
 

Abstract:

IMMOBILIZED METAL AFFINITY CHROMATOGRAPHY (IMAC) HAS SUBTANTIAL UTILITY AS A PROTEIN PURIFICATION TOOL FOR BOTH ANALYTICAL AND PREPARATIVE APPLICATIONS. THERE IS A STRONG MARKET NEED FOR AN IMAC SUPPORT SUITABLE FOR HIGH PERFORMANCE LIQUID CHROMATOGRAPHY (HPLC). RESEARCHERS ARE DEVELOPING AN HPLC SUPPORT WITH GOOD PH STABILITY, LOW NONSPECIFIC BINDING, GOOD CAPACITY, DURABILITY, AND RAPID ADSORPTION KINETICS. CAPTURE LIGANDS ARE IMMOBILIZED ON LARGE PARTICLE SILICA VIA A 50 A HYDROPHILIC TETHER. THIS RESEARCH COMBINES THE HPLC AFFINITY SUPPORT WITH WELL CHARACTERIZED AFFINITY PROBES FOR IMAC OF PROTEINS. RESEARCHERS ARE PREPARING AND CHARACTERIZING TWO NEW IMAC SUPPORTS FOR HPLC, MEASURING THE PROTEIN ADSORPTION CAPACITY FOR MODEL PROTEINS UNDER VARIOUS CONDITIONS, AND DETERMINING RESOLUTION OF CLOSELY RELATED PROTEIN ON THE COLUMNS. THESE STUDIES DEFINE THE UTILITY OF IMMOBILIZING IMAC AFFINITY PROBES ON HYDROPHILIC TETHERS FOR RAPID, HIGH RESOLUTION PROTEIN PURIFICATION.

Principal Investigator:

Holly A Thompson
4067285897

Business Contact:

Small Business Information at Submission:

Chromatochem Inc.
2837 Ft Missoula Rd Missoula, MT 59801

EIN/Tax ID:
DUNS: N/A
Number of Employees: N/A
Woman-Owned: No
Minority-Owned: No
HUBZone-Owned: No