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ENHANCED ELISA PERFORMANCE USING ACOUSTIC MICROSTREAMING

Award Information

Agency:
Department of Health and Human Services
Branch:
N/A
Award ID:
11074
Program Year/Program:
1991 / SBIR
Agency Tracking Number:
11074
Solicitation Year:
N/A
Solicitation Topic Code:
N/A
Solicitation Number:
N/A
Small Business Information
Chromogen, Inc
P O Box 128 Milton, VT 05468
View profile »
Woman-Owned: No
Minority-Owned: No
HUBZone-Owned: No
 
Phase 2
Fiscal Year: 1991
Title: ENHANCED ELISA PERFORMANCE USING ACOUSTIC MICROSTREAMING
Agency: HHS
Contract: N/A
Award Amount: $500,000.00
 

Abstract:

SOLID-PHASE, ENZYME-LINKED IMMUNOSORBENT ASSAYS (ELISA'S), SUCH AS THE ELISA AIDS SCREENING TEST, DEPEND ON THE DIFFUSION OF MACROMOLECULES AND ORGANIC SUBSTRATES ACROSS A QUIESCENT LIQUID BOUNDARY LAYER PRIOR TO ADSORPTION TO THE SOLID PHASE OR REACTIVITY WITH SOLID-PHASE-BOUND MACROMOLECULES. TIME-DEPENDENT DIFFUSION OFTEN RESULTS IN LENGTHY INCUBATION TIMES, OR ELEVATED CONCENTRATIONS OF EXPENSIVE OR RARE REAGENTS TO REDUCE INCUBATION TIME. STIRRING BY VIGOROUS MICROPLATE AGITATION IS ONLY MARGINALLYEFFECTIVE IN IMPROVING DIFFUSION, CANNOT BE DONE WHILE THE ENZYME-SUBSTRATE REACTION IS READ IN FAST KINETIC APPLICATIONS, AND PRESENTS POTENTIAL BIOHAZARD AND INSTRUMENT MALFUNCTION PROBLEMS. THE GOAL OF THIS RESEARCH IS TO DETERMINE THE EFFICACY OF ACOUSTIC MICROSTREAMING FOR IMPROVING ELISA PERFORMANCE. THE AIMS ARE: (1) TO USE AN EXISTING ACOUSTIC MICROSTREAMINGPROTOTYPE DEVICE TO DETERMINE OPTIMUM CONDITIONS TO ENHANCE BINDING OF ELISA IMMUNOREACTANTS TO MICROPLATE WELLS, BOTH AT ROOM AND ELEVATED TEMPERATURES; AND (2) TO DETERMINE THE VALUE OF ACOUSTICALLY ENHANCED ELISA PERFORMANCE AS MEASUREDBY INCREASED SENSITIVITY, DECREASED INCUBATION TIME, AND DECREASED REAGENT EXPENDITURE. THE LONG-TERM OBJECTIVE (PHASE II) IS TO DEVELOP AN ACOUSTICMICROPLATE ACOUSTIC MICROSTREAMING DEVICE WITH ELEVATED TEMPERATURE INCUBATOR.

Principal Investigator:


0

Business Contact:

Small Business Information at Submission:

Chromogen Inc.
3-19 Chace Mill Bldg 1 Mill St Burlington, VT 05401

EIN/Tax ID:
DUNS: N/A
Number of Employees: N/A
Woman-Owned: No
Minority-Owned: No
HUBZone-Owned: No