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Protein Microarrays for Bioreactor Bioproduct Monitoring

Award Information

Agency:
National Aeronautics and Space Administration 		Branch:
N/A 		Award ID:
56527 		Program Year/Program:
2002 / SBIR
Agency Tracking Number:
012983 		Solicitation Year:
N/A 		Solicitation Topic Code:
N/A 		Solicitation Number:
N/A
Small Business Information
CIENCIA, INC.
111 ROBERTS ST, STE K EAST HARTFORD, CT 06108-
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Woman-Owned: No
Minority-Owned: No
HUBZone-Owned: No
 
Phase 1
Fiscal Year: 2002
Title: Protein Microarrays for Bioreactor Bioproduct Monitoring
Agency: NASA
Contract: NAS9-02079
Award Amount: $70,000.00
 

Abstract:

Rotating wall vessels (RWV) are widely used for cell culture in simulated microgravity conditions. Sensor technologies capable of monitoring the expression of proteins on a large scale (proteomics) are needed to fully exploit the unique capabilities of RWVs. This Phase II effort is part of a program to develop an advanced analytical monitoring system for quantitative detection of bioreactor bioproducts in microgravity. The underlying technology is grating-coupled surface plasmon resonance, which permits sensitive, label-free, parallel detection of bioproducts in a complex fluid without purification steps in real time. The objective of this effort is to develop generic capture microarrays that will allow for the site-directed self-assembly of tailor-made, user-defined high-density multi-component assay chips. This is accomplished by immobilizing a library of short oligonucleotide PNA (peptide nucleic acid) probe molecules on a chip in a site-specific manner using covalent attachment chemistry and a spotting method. Capture molecules that will compose the array are tagged (off-line) with a library of complementary PNA sequences. Then, the microarray is assembled by flowing the complex mixture of different PNA-tagged compounds over the chip, which through hybridization reactions, form site-directed self-assembled arrays. A set of microarrays will be produced and a complementary set of protein-PNA conjugates relevant to microgravity research.

Principal Investigator:

Salvador M. Fernandez
Principal Investigator
8605289737
fernandez@ciencia.com

Business Contact:

Arturo Pilar
Business Official
8605289737
apilar@ciencia.com
Small Business Information at Submission:

Ciencia, Inc.
111 Roberts Street, Suite K East Hartford, CT 06108

EIN/Tax ID: 631009790
DUNS: N/A
Number of Employees:
Woman-Owned: No
Minority-Owned: No
HUBZone-Owned: No