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ANALYSIS OF YEAST GLYCOSYLATION OF A HUMAN GLYCOPROTEIN

Award Information

Agency:
Department of Health and Human Services
Branch:
N/A
Award ID:
3188
Program Year/Program:
1987 / SBIR
Agency Tracking Number:
3188
Solicitation Year:
N/A
Solicitation Topic Code:
N/A
Solicitation Number:
N/A
Small Business Information
C-MOTION, INC.
C-MOTION, INC. 15821A CRABBS BRANCH WAY ROCKVILLE, MD 20855
View profile »
Woman-Owned: No
Minority-Owned: No
HUBZone-Owned: No
 
Phase 2
Fiscal Year: 1987
Title: ANALYSIS OF YEAST GLYCOSYLATION OF A HUMAN GLYCOPROTEIN
Agency: HHS
Contract: N/A
Award Amount: $227,000.00
 

Abstract:

WE PROPOSE TO USE THE GENE FOR ALPHA-1-ANTI-TRYPSIN (1-AT), A MODERATELY GLYCOSYLATED HUMAN SERUM PROTEIN, AS A SPECIFIC MODEL TO TEST THE FEASIBILITY OF USING BAKERS' YEAST (S. CEREVISIAE) AS A HOST MICROORGANISM FOR PRODUCTION OF HUMAN GLYCOPROTEINS WITH PHARMACEUTICAL APPLICATIONS. SPECIFICALLY, WE INTEND TO CONSTRUCT YEAST EXPRESSION VECTORS TO BOTH PRODUCE 1-AT IN THE CYTOPLASM AND TO DIRECT IT INTO THE SECRETION PATHWAY WHERE IT MAY BE GLYCOSYLATED. THE EXPERIMENTS WILL BE DESIGNED TO ANSWER THE QUESTION, CAN THE YEAST CELL ADD CARBOHYDRATE TO THE CORRECT ASPARAGINE RECEPTORS OF A MAMMALIAN GLYCOPROTEIN? ASSUMING THAT ANSWER IS AFFIRMATIVE, IN PHASE II THE SERUM STABILITY OF DIFFERENT FORMS OF 1-AT, PRODUCED IN PHASE I BY SPECIFIC YEAST MUTANTS WHICH ADD DIFFERENT PATTERNS OF CARBOHYDRATE, WILL BE COMPARED WITH ONE ANOTHER AND WITH UNGLYCOSYLATED 1-AT BY MEANS OF A RAT MODEL SYSTEM. ALSO IN PHASE II, ADDITIONAL CARBOHYDRATE PATTERNS WILL BE GENERATED BY IN VITRO ENZYMATIC METHODS APPLIED TO A SUITABLE INTERMEDIATE DERIVED FROM A PARTICULAR YEAST MUTANT WHICH ADDS A SHORTENED CARBOHYDRATE CORE TO PROTEINS. THESE EXPERIMENTS ARE AIMED AT DISCOVERING A METHOD TO PRODUCE HUMAN GLYCOPROTEINS IN A MICROORGANISM BY EXPLORING WAYS TO OVERCOME THE POTENTIAL PROBLEMS OF STABILITY AND ANTIGENICITY.

Principal Investigator:

Donald Taylor Moir
Principal Investigator
6178619700

Business Contact:

Small Business Information at Submission:

Collaborative Research Inc.
128 Spring Street Lexington, MA 02173

EIN/Tax ID: 522053087
DUNS: N/A
Number of Employees: N/A
Woman-Owned: No
Minority-Owned: No
HUBZone-Owned: No