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THE OBJECTIVE OF THIS RESEARCH PLAN IS TO ISOLATE A CDNA SEQUENCE ENCODING…

Award Information

Agency:
Department of Health and Human Services
Branch:
N/A
Award ID:
3032
Program Year/Program:
1985 / SBIR
Agency Tracking Number:
3032
Solicitation Year:
N/A
Solicitation Topic Code:
N/A
Solicitation Number:
N/A
Small Business Information
C-MOTION, INC.
C-MOTION, INC. 15821A CRABBS BRANCH WAY ROCKVILLE, MD 20855
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Woman-Owned: No
Minority-Owned: No
HUBZone-Owned: No
 
Phase 1
Fiscal Year: 1985
Title: THE OBJECTIVE OF THIS RESEARCH PLAN IS TO ISOLATE A CDNA SEQUENCE ENCODING TUMOR NECROSIS FACTOR (TNF) AND, BY MANIPULATION OF THAT SEQUENCE, TO PRODUCE AND SECRETE THE MATURE TNF PROTEIN FROM THE YEAST SACCHAROMYCES CEREVISIAE.
Agency: HHS
Contract: N/A
Award Amount: $50,000.00
 

Abstract:

THE OBJECTIVE OF THIS RESEARCH PLAN IS TO ISOLATE A CDNA SEQUENCE ENCODING TUMOR NECROSIS FACTOR (TNF) AND, BY MANIPULATION OF THAT SEQUENCE, TO PRODUCE AND SECRETE THE MATURE TNF PROTEIN FROM THE YEAST SACCHAROMYCES CEREVISIAE. PREVIOUS WORK ON TNF HAS SHOWN IT TO HAVE CYTOSTATIC OR CYTOLYTIC ACTIVITY IN VITRO AGAINST A NUMBER OF TRANSFORMED CELL LINES AND TO HAVE TUMOR NECROTIC ACTIVITY AGAINST VARIOUS TUMORS IN ANIMAL MODELS. MOST IMPORTANTLY, IT HAS NO ADVERSE EFFECT ON NORMAL CELLS EITHER IN CULTURE OR IN ANIMAL MODELS. THIS SPECIFICITY FOR TUMOR CELLS SUGGESTS A POTENTIAL VALUABLE THERAPEUTIC ROLE FOR TNF IN TREATING HUMAN TUMORS. IN ORDER TO ESTABLISH ITS VALUE, HOWEVER, SUFFICIENT QUANTITIES OF THIS RATHER RARE PROTEIN MUST BE ISOLATED FOR TESTING. THE OVERALL AIM OF THIS RESEARCH PROPOSAL IS TO PRODUCE TNF ECONOMICALLY IN LARGE QUANTITIES FROM YEAST CELLS. AS A RESULT OF THE PHASE I EFFORTS DESCRIBED HERE, A CDNA COPY OF THE TNF GENE WILL BE ISOLATED AND CHARACTERIZED. IN PHASE II, THE PORTION OF THE GENE CODING FOR MATURE TNF WILL BE ATTACHED TO YEAST PROMOTER AND SECRETION SIGNAL SEQUENCES, AND THE ENTIRE TRANSCRIPTIONAL UNIT WILL BE INTEGRATED STABLY INTO THE YEAST GENOME. THE RESULT OF PHASE II SHOULD BE A GENETICALLY STABLE YEAST STRAIN SECRETING 1020 MG OF TNF PER LITER OF MEDIUM.

Principal Investigator:

Donald taylor moir
PRINCIPAL INVESTIGATOR
6178619700

Business Contact:

Small Business Information at Submission:

Collaborative Research Inc
128 Spring Street Lexington, MA 02173

EIN/Tax ID: 522053087
DUNS: N/A
Number of Employees: N/A
Woman-Owned: No
Minority-Owned: No
HUBZone-Owned: No