USA flag logo/image

An Official Website of the United States Government

WE PROPOSE TO DEVELOP A METHODOLOGY BY WHICH THE GENES CODING FOR AUTOIMMUNE…

Award Information

Agency:
Department of Health and Human Services
Branch:
N/A
Award ID:
2910
Program Year/Program:
1985 / SBIR
Agency Tracking Number:
2910
Solicitation Year:
N/A
Solicitation Topic Code:
N/A
Solicitation Number:
N/A
Small Business Information
C-MOTION, INC.
C-MOTION, INC. 15821A CRABBS BRANCH WAY ROCKVILLE, MD 20855
View profile »
Woman-Owned: No
Minority-Owned: No
HUBZone-Owned: No
 
Phase 1
Fiscal Year: 1985
Title: WE PROPOSE TO DEVELOP A METHODOLOGY BY WHICH THE GENES CODING FOR AUTOIMMUNE DISEASE-SPECIFIC PROTEIN ANTIGENS CAN BE RAPIDLY CLONED USING RECOMBINANT DNA TECHNIQUES.
Agency: HHS
Contract: N/A
Award Amount: $50,000.00
 

Abstract:

WE PROPOSE TO DEVELOP A METHODOLOGY BY WHICH THE GENES CODING FOR AUTOIMMUNE DISEASE-SPECIFIC PROTEIN ANTIGENS CAN BE RAPIDLY CLONED USING RECOMBINANT DNA TECHNIQUES. THE SYSTEM WHICH WE ARE USING LENDS ITSELF TO HIGH-LEVEL EXPRESSION OF THESE ANTIGENS AS HYBRID PROTEINS IN E. COLI. THIS FACILITATES PRODUCTION OF THESE ANTIGENS FOR IN PHASE I A CDNA LIBRARY WILL BE CONSTRUCTED FROM HUMAN THYROID TISSUE. CLONING WILL BE INTO THE LAMBDA GT11 SYSTEM. CLONES CARRYING GRAVES-SPECIFIC SEQUENCES WILL BE IDENTIFIED BY DIFFERENTIAL IMMUNOSCREENING WITH SERA FROM GRAVES' PATIENTS AND SERA FROM NORMAL INDIVIDUALS. GRAVES' POSITIVE CLONES CAN BE INDUCED WITH THE LAMBDA GT11 SYSTEM, LEADING TO EXPRESSION OF SUBSTANTIAL AMOUNTS OF THE GRAVES' ANTIGEN FUSED TO BETA-GALACTOSIDASE (HYBRID PROTEIN). BECAUSE OF THEIR LARGE SIZE, THEY CAN SUBSEQUENTLY BE PURIFIED EASILY. IN PHASE II THE CLONED GRAVES' ANTIGENS WILL BE CHARACTERIZED. THEY WILL BE TESTED EXTENSIVELY FOR THE ABILITY TO BIND SPECIFICALLY WITH AUTOANTIBODIES PRESENT IN THE SERA FROM GRAVES' PATIENTS. CLONED ANTIGENS DETERMINED TO BE GRAVES-SPECIFIC WILL BE USED TO DEVELOP DIAGNOSTIC

Principal Investigator:

Donald warren bowden
PRINCIPAL INVESTIGATOR
6178619700

Business Contact:

Small Business Information at Submission:

Collaborative Research Inc
128 Spring Street Lexington, MA 02173

EIN/Tax ID: 522053087
DUNS: N/A
Number of Employees: N/A
Woman-Owned: No
Minority-Owned: No
HUBZone-Owned: No