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PCR DEVELOPMENT AND LOCALIZATION OF CRI RFLP PROBES

Award Information

Agency:
Department of Health and Human Services
Branch:
N/A
Award ID:
16670
Program Year/Program:
1993 / SBIR
Agency Tracking Number:
16670
Solicitation Year:
N/A
Solicitation Topic Code:
N/A
Solicitation Number:
N/A
Small Business Information
C-MOTION, INC.
C-MOTION, INC. 15821A CRABBS BRANCH WAY ROCKVILLE, MD 20855
View profile »
Woman-Owned: No
Minority-Owned: No
HUBZone-Owned: No
 
Phase 2
Fiscal Year: 1993
Title: PCR DEVELOPMENT AND LOCALIZATION OF CRI RFLP PROBES
Agency: HHS
Contract: N/A
Award Amount: $500,000.00
 

Abstract:

HUMAN RFLP PROBES WHICH HAVE BEEN GENETICALLY MAPPED WILL BE CONVERTED INTO PCR-BASED MARKERS. MAPPED RFLP PROBES ARE AN IMPORTANT TOOL FOR RESEARCH IN MANY AREAS. A SUBSET OF ABOUT 150 RFLP PROBES HAS BEEN SELECTED AND IS CURRENTLY BEING USED FOR MAPPING GENETIC DISEASE GENES AND STUDYING LOSS OF HETEROZYGOSITY IN TUMOR CELLS. CONVERTING THE SUBSET PROBES INTO PCR-BASED MARKERS WILL GREATLY FACILITATE THESE STUDIES BECAUSE PCR-BASED GENOTYPING METHODS ARE A SIGNIFICANT IMPROVEMENT OVER CURRENTLY USED GENOMIC SOUTHERN HYBRIDIZATION METHODS. THE OLIGONUCLEOTIDES DEVELOPED IN THIS RESEARCH WILL ALSO BE AN IMPORTANT RESEARCH PRODUCT FOR CONSTRUCTING PHYSICAL MAPS FOR HUMAN CHROMOSOMES. IN SOME CASES THEY CAN ALSO BE USED FOR THE DIAGNOSIS OF GENETIC DISEASES AND TUMORS, AND FOR IDENTITY TESTING. IN PHASE I FEASIBILITY STUDIES, TWO PCR-MARKERS INVOLVING CA DINUCLEOTIDE REPEATS, WHICH ARE IN THE VICINITY OF THE ORIGINALLY MAPPED RFLP LOCI, WILL BE DEVELOPED. IN ADDITION, THE REGION FLANKING A DIMORPHIC RFLP WILL BE SUBCLONED AND SEQUENCED USING A RANDOM APPROACH. IN PHASE II, PCRS WILL BE DEVELOPED FOR THE ENTIRE SUBSET OF RFLP PROBES. OTHER METHODS SUCH AS DGGE (DENATURING GRADIENT GEL ELECTROPHORESIS), SSCP (SINGLE-STRANDED CONFORMATION POLYMORPHISM), INVERSE PCR AND VECTORETTE PCR WILL ALSO BE USED TO ACHIEVE THE OVERALL GOAL.

Principal Investigator:

Mao, Jen-i
Principal Investigator
6172750004

Business Contact:

1 r43 hg00491-01
Small Business Information at Submission:

Collaborative Research, Inc.
Two Oak Park Bedford, MA 01730

EIN/Tax ID: 522053087
DUNS: N/A
Number of Employees: N/A
Woman-Owned: No
Minority-Owned: No
HUBZone-Owned: No