USA flag logo/image

An Official Website of the United States Government

ALLELE-SPECIFIC MULTIPLEX DNA DIAGNOSTIC TEST

Award Information

Agency:
Department of Health and Human Services
Branch:
N/A
Award ID:
16616
Program Year/Program:
1993 / SBIR
Agency Tracking Number:
16616
Solicitation Year:
N/A
Solicitation Topic Code:
N/A
Solicitation Number:
N/A
Small Business Information
C-MOTION, INC.
C-MOTION, INC. 15821A CRABBS BRANCH WAY ROCKVILLE, MD 20855
View profile »
Woman-Owned: No
Minority-Owned: No
HUBZone-Owned: No
 
Phase 2
Fiscal Year: 1993
Title: ALLELE-SPECIFIC MULTIPLEX DNA DIAGNOSTIC TEST
Agency: HHS
Contract: N/A
Award Amount: $500,000.00
 

Abstract:

THE AIM OF THIS PROJECT IS TO DEMONSTRATE A NEW MULTIPLEX DNA DIAGNOSTIC TEST WHICH CAN SIMULTANEOUSLY DETECT NUCLEOTIDE DIFFERENCES BETWEEN SEVERAL DIFFERENT ALLELES AT SEVERAL DIFFERENT GENETIC LOCI IN A SINGLE TEST SAMPLE OF HUMAN DNA. THE TEST PORTION OF THE PROTOCOL RELIES ON ALLELE-SPECIFIC POLYMERASE CHAIN REACTIONS (PCR) TO PRODUCE AMPLIFIED PRODUCTS ONLY WHEN THE PRIMER SEQUENCES ARE COMPLEMENTARY TO THE LOCUS BEING TESTED. MANY OF THESE ALLELE-SPECIFIC PCRS WILL BE CARRIED OUT SIMULTANEOUSLY ON A SINGLE TEST SAMPLE OF DNA. THE EXISTENCE OF AMPLIFICATION PRODUCTS FROM THE VARIOUS PRIMERS WILL THEN BE DETERMINED BY MULTIPLEX CAPTURE OF THE PRODUCTS ON A SOLID SUPPORT BY HYBRIDIZATION BETWEEN SPECIFIC OLIGONUCLEOTIDES WHICH WERE PREVIOUSLY ATTACHED TO THE SUPPORT. RESULTS WILL BE READ BY THE PRESENCE OR ABSENCE OF LABELED AMPLIFICATION PRODUCTS AT EACH SPOT ON THE SUPPORT. LABELS WILL BE NONRADIOACTIVE SUCH AS BIOTIN-STREPTAVIDIN-HORSERADISH PEROXIDASE. HYBRIDIZATION TO THE TAIL COMPLEMENTS ON THE SOLID SUPPORT WILL BE BY CONVENTIONAL WATSON-CRICK HYDROGEN BONDING OR BY HOOGSTEEN BONDING IN A TRIPLE HELIX CONFIGURATION. IN THE LATTER CASE, NO DENATURATION OF THE PCR PRODUCTS WILL BE REQUIRED PRIOR TO HYBRIDIZATION. IN THIS NEW TEST, ALLELE-SPECIFIC PCR AMPLIFICATIONS WILL BE PERFORMED IN BATCHES OF ABOUT TEN PER DNA SAMPLE, BUT THE TEST IS EXPECTED TO PERMIT SIMULTANEOUS CAPTURE AND SELECTION OF PRODUCTS FROM HUNDREDS OF ALLELES ON A SINGLE SOLID SUPPORT.

Principal Investigator:

Moir, Donald T
Principal Investigator
6172750004

Business Contact:

1 r43 gm43697-01a1"
Small Business Information at Submission:

Collaborative Research, Inc.
Two Oak Park Bedford, MA 01730

EIN/Tax ID: 522053087
DUNS: N/A
Number of Employees: N/A
Woman-Owned: No
Minority-Owned: No
HUBZone-Owned: No