A Set of Mapping Panels for Localizing Human Genetic Diseases
The objective of this proposal is to develop and distribute a set of mapping panels for all human autosomes and the X chromosome. These mapping panels, consisting of 300-400 highly polymorphic loci spaced at 10-15 centiMorgan (cM) intervals, will provide the necessary tools for an efficient, and cost effective, search of the genome for markers linked to human diseases. With recent progress in the development of microsatellite markers that can be typed using the polymerase chain reaction (PCR), a large number of high quality markers will soon be available for use in disease gene mapping. A subset of these that will be sufficient to span a chromosome and will be robust for the collection of genotypic data will be selected for the mapping panels. During Phase I, mapping panels for human chromosomes 10 and 20, consisting of 17-25 and 10-15 markers, respectively, will be developed. Markers with heterozygosities = 0.70 will be selected at 10-15 cM intervals based on their reliability and efficiency of use, following a thorough PCR evaluation. Multipoint maps, including loci in the mapping subset and higher resolution maps, will be constructed.
Small Business Information at Submission:
Principal Investigator:Tim P. Keith
Collaborative Research, Inc.
1365 Main Street Waltham, MA 02154
Number of Employees: