USA flag logo/image

An Official Website of the United States Government

P53 Mutation Screening by Multiplex Sequencing

Award Information

Agency:
Department of Health and Human Services
Branch:
N/A
Award ID:
24901
Program Year/Program:
1994 / SBIR
Agency Tracking Number:
24901
Solicitation Year:
N/A
Solicitation Topic Code:
N/A
Solicitation Number:
N/A
Small Business Information
C-MOTION, INC.
C-MOTION, INC. 15821A CRABBS BRANCH WAY ROCKVILLE, MD 20855
View profile »
Woman-Owned: No
Minority-Owned: No
HUBZone-Owned: No
 
Phase 1
Fiscal Year: 1994
Title: P53 Mutation Screening by Multiplex Sequencing
Agency: HHS
Contract: 1 R43 CA65204-1,
Award Amount: $81,000.00
 

Abstract:

We will develop a rapid, cost-effective screening method for detecting all mutations in the p53gene. The goal of Phase I is to optimize conditions for direct genomic sequencing of PCR products fromhuman tumor cell lines. Both malignant mesotheliomas and sarcomas have been shown to be inducedby different chemical carcinogens. Forty malignant mesothelioma and sarcoma cell lines, for whichmutations have previously been detected, will be studied. These samples will allow the determinationof the efficiency and applicability of multiplex sequencing for scanning genomic DNA for point mutations.Phase II will involve the study of large numbers of malignant mesotheliomas. These experiments willdetermine if specific codons or exons in p53 are mutated in malignant mesotheliomas and if there is anycorrelation between the site of mutation and the clinical outcome. Overall, this research willdemonstrate the feasibility of using a multiplex sequencing approach for mutation scanning in humanclinical samples. While the specific aims of this project are to determine the location of p53 mutationsin malignant mesotheliomas, the methods developed will allow one to screen other forms of cancer formutations in p53 as well as in other genes.

Principal Investigator:

Lynn Doucette-Stamm
6174877979

Business Contact:

Small Business Information at Submission:

Collaborative Research, Inc.
1365 Main Street Waltham, MA 02154

EIN/Tax ID: 522053087
DUNS: N/A
Number of Employees: N/A
Woman-Owned: No
Minority-Owned: No
HUBZone-Owned: No