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SYNTHESIS OF FLUORESCENT INDICATORS FOR CYTOSOLIC CA2+

Award Information

Agency:
Department of Health and Human Services
Branch:
N/A
Award ID:
4710
Program Year/Program:
1988 / SBIR
Agency Tracking Number:
4710
Solicitation Year:
N/A
Solicitation Topic Code:
N/A
Solicitation Number:
N/A
Small Business Information
COVALENT ASSOC., INC.
541 NW 11th Street Corvallis, OR 97330 6018
View profile »
Woman-Owned: No
Minority-Owned: No
HUBZone-Owned: No
 
Phase 2
Fiscal Year: 1988
Title: SYNTHESIS OF FLUORESCENT INDICATORS FOR CYTOSOLIC CA2+
Agency: HHS
Contract: N/A
Award Amount: $461,000.00
 

Abstract:

A SERIES OF IMPROVED FLUORESCENT DYES SPECIFIC FOR COMPLEXING CA2+ WILL BE SYNTHESIZED AND EVALUATED FOR THEIR ABSORPTION AND EMISSION CHARACTERISTICS AND THEIR ABILITY TOBIND CA2+. THESE CHELATORS INCORPORATE BENZOCOUMARIN LASER DYE FLUOROPHORES, WHICH HAVE DESIRABLE HIGH-QUANTUM YIELDS AND VISIBLE WAVELENGTH ABSORPTION AND EMISSION. THE FLUOROPHORE IS ATTACHED TO AN EGTA-LIKE (ETHYLENEGLYCOL BIS [B-AMINO ETHYL ETHER N,N,N',N'-TETRAACETIC ACID]) STRUCTURE THAT IS KNOWN TO HAVE GREAT SPECIFICITY FOR CA2+ OVER MG2+ AND OTHER DIVALENT METAL IONS. THE PROPOSED CA2+ INDICATORSWILL HAVE IMPROVED OPTICAL CHARACTERISTICS OVER THOSE CURRENTLY USED (QUIN 2 AND FURA-2). THE NEW INDICATORS WILLBE CELL MEMBRANE PERMEABLE WHEN THE ACETATE GROUPS ARE ESTERIFIED, AND RECONVERTIBLE TO THE ACETATE FORM BY CYTOSOLIC ESTERASES INSIDE THE CELL. THUS THE TRAPPED CHELATOR CAN BE USED TO FOLLOW CONCENTRATION CHANGES IN INTRACELLULAR CALCIUM CONCENTRATION AS A FUNCTION OF TIME INRESPONSE TO VARIOUS PERTURBING INFLUENCES. INTRACELLULAR CALCIUM IS CURRENTLY THE SUBJECT OF WIDESPREAD INTEREST AS ASECOND MESSENGER THAT TRIGGERS AND REGULATES CELL ACTIVATION. THIS PHASE I WORK INVOLVES SYNTHESIS OF THE SUBSTITUTED BENZOCOUMARIN PORTION OF THE OVERALL STRUCTURE. IN PHASE II, THE FLUOROPHORES WILL BE LINKED TO THE EGTA- LIKE COMPLEXING AGENT. THE RESULTING SERIES OF FLUORESCENT CA2+ COMPLEXING AGENTS WILL BE EVALUATED FOR THEIR ABSORPTION AND EMISSION WAVELENGTHS AND FLUORESCENCE QUANTUM YIELD IN THE FREE AND CA2+-BOUND STATES. IN ADDITION, THEIR CA2+ COMPLEX STABILITY CONSTANTS WILL BE DETERMINED. THE MOST PROMISING INDICATORS WILL BE TESTED FOR CA2+ SPECIFICITY AND USEFULNESS INDICATORS OF INTRACELLULAR CONCENTRATION.

Principal Investigator:

Dr Margaret E Langmuir
Principal Investigator
6179381140

Business Contact:

Small Business Information at Submission:

Covalent Associates Inc.
52 Dragon Court Woburn, MA 01801

EIN/Tax ID:
DUNS: N/A
Number of Employees: N/A
Woman-Owned: No
Minority-Owned: No
HUBZone-Owned: No