SYNTHESIS OF NEW FLUOROPHORES FOR CYTOSOLIC GLUTATHIONE

Award Information

Agency: Department of Health and Human Services	Branch: N/A	Award ID: 7280	Program Year/Program: 1987 / SBIR
Agency Tracking Number: 7280	Solicitation Year: N/A	Solicitation Topic Code: N/A	Solicitation Number: N/A

Small Business Information: COVALENT ASSOC., INC.
541 NW 11th Street Corvallis, OR 97330 6018
View profile »

Woman-Owned: No

Minority-Owned: No

HUBZone-Owned: No

Phase 1: Fiscal Year: 1987

Title: SYNTHESIS OF NEW FLUOROPHORES FOR CYTOSOLIC GLUTATHIONE

Agency: HHS

Contract: N/A

Award Amount: $48,000.00

Abstract:
COVALENT ASSOCIATES, INC. PROPOSES THE SYNTHESIS OF A NEW FLUOROPHORE FOR INTRACELLULAR GLUTATHIONE DETERMINATION. THE REACTION PRODUCT OF THE FLUOROPHORE WITH THIOLS WILL HAVE LONGER WAVELENGTH ADSORPTION AND EMISSION AND HIGHER QUANTUM YIELD THAN THE BEST CYTOSOLIC THIOL FLUROPHORE CURRENTLY IN USE FOR CELL SORTING BY FLOW CYTOMETRY. THE DYE WILL HAVE THE ADVANTAGE OF BEING CELL-MEMBRANE-PERMEABLE OWING TO ESTERIFIED PHENOL AND CARBOXYL GROUPS WHICH WILL SUBSEQUENTLY BE DE-ESTERFIED BY CYTOSOLIC ESTERASES, THUS TRAPPING THE INDICATOR WITHIN THE CELL. THEREFORE, CELL LOADING SHOULD OCCUR AT MUCH LOWER EXTRACELLULAR DYE CONCENTRATIONS THAN CURRENTLY POSSIBLE. ADSORPTION AND EMISSION PROPERTIES IN BOTH BOUND AND UNBOUNDSTATES WIT GLUTATHIONE AND THIOL-CONTAINING PROTEINS WILL ALSO BE STUDIED.

Principal Investigator:
Dr. margaret e lagmuir
6179381140

Business Contact:

Small Business Information at Submission:

Covalent Associates Inc
52 Dragon Court Woburn, MA 01801

EIN/Tax ID:

DUNS: N/A

Number of Employees: N/A

Woman-Owned: No

Minority-Owned: No

HUBZone-Owned: No