FLOURESCENT THIOL PROBES -- CYTOMETRY AND PCR TECHNOLOGY

Award Information

Agency: Department of Health and Human Services	Branch: N/A	Award ID: 19066	Program Year/Program: 1994 / SBIR
Agency Tracking Number: 19066	Solicitation Year: N/A	Solicitation Topic Code: N/A	Solicitation Number: N/A

Small Business Information: COVALENT ASSOC., INC.
541 NW 11th Street Corvallis, OR 97330 6018
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Woman-Owned: No

Minority-Owned: No

HUBZone-Owned: No

Phase 2: Fiscal Year: 1994

Title: FLOURESCENT THIOL PROBES -- CYTOMETRY AND PCR TECHNOLOGY

Agency: HHS

Contract: N/A

Award Amount: $521,000.00

Abstract:
WE WILL SYNTHESIZE NEW PROBES FOR THE DETERMINATION OF INTRACELLULAR GLUTATHIONE AND OTHER CELLULAR THIOLS. THE REACTION PRODUCT OF THE PROBES WITH THIOLS WILL HAVE LONGER WAVELENGTH ABSORPTION AND EMISSION AND HIGHER QUANTUM YIELD THAN THE BEST CYTOSOLIC THIOL FLUOROPHORES CURRENTLY IN USE FOR CELL SORTING BY FLOW CYTOMETRY. THE DYES WILL HAVE THE ADVANTAGE OF BEING CELL MEMBRANE PERMEABLE SO THAT CELL LOADING CAN OCCUR AT LOWER EXTRA CELLULAR DYE CONCENTRATIONS THAN CURRENTLY POSSIBLE. THE SYNTHESIZED PROBES WILL BE TESTED IN VITRO AND IN CELL CULTURES FOR ABSORPTION AND EMISSION PROPERTIES WHEN BOUND TO GLUTATHIONE AND OTHER PROTEIN THIOLS. CELL TOXICITY WILL ALSO BE DETERMINED.

Principal Investigator:
Margaret Langmuir
6179381140

Business Contact:

Small Business Information at Submission:

Covalent Associates Inc.
10 State Street Woburn, MA 01801

EIN/Tax ID:

DUNS: N/A

Number of Employees: N/A

Woman-Owned: No

Minority-Owned: No

HUBZone-Owned: No