USA flag logo/image

An Official Website of the United States Government

FLOURESCENT THIOL PROBES -- CYTOMETRY AND PCR TECHNOLOGY

Award Information

Agency:
Department of Health and Human Services
Branch:
N/A
Award ID:
19066
Program Year/Program:
1994 / SBIR
Agency Tracking Number:
19066
Solicitation Year:
N/A
Solicitation Topic Code:
N/A
Solicitation Number:
N/A
Small Business Information
COVALENT ASSOC., INC.
541 NW 11th Street Corvallis, OR 97330 6018
View profile »
Woman-Owned: No
Minority-Owned: No
HUBZone-Owned: No
 
Phase 2
Fiscal Year: 1994
Title: FLOURESCENT THIOL PROBES -- CYTOMETRY AND PCR TECHNOLOGY
Agency: HHS
Contract: N/A
Award Amount: $521,000.00
 

Abstract:

WE WILL SYNTHESIZE NEW PROBES FOR THE DETERMINATION OF INTRACELLULAR GLUTATHIONE AND OTHER CELLULAR THIOLS. THE REACTION PRODUCT OF THE PROBES WITH THIOLS WILL HAVE LONGER WAVELENGTH ABSORPTION AND EMISSION AND HIGHER QUANTUM YIELD THAN THE BEST CYTOSOLIC THIOL FLUOROPHORES CURRENTLY IN USE FOR CELL SORTING BY FLOW CYTOMETRY. THE DYES WILL HAVE THE ADVANTAGE OF BEING CELL MEMBRANE PERMEABLE SO THAT CELL LOADING CAN OCCUR AT LOWER EXTRA CELLULAR DYE CONCENTRATIONS THAN CURRENTLY POSSIBLE. THE SYNTHESIZED PROBES WILL BE TESTED IN VITRO AND IN CELL CULTURES FOR ABSORPTION AND EMISSION PROPERTIES WHEN BOUND TO GLUTATHIONE AND OTHER PROTEIN THIOLS. CELL TOXICITY WILL ALSO BE DETERMINED.

Principal Investigator:

Margaret Langmuir
6179381140

Business Contact:

Small Business Information at Submission:

Covalent Associates Inc.
10 State Street Woburn, MA 01801

EIN/Tax ID:
DUNS: N/A
Number of Employees: N/A
Woman-Owned: No
Minority-Owned: No
HUBZone-Owned: No