USA flag logo/image

An Official Website of the United States Government

ENGINEERING OF ANTIBODY BINDING SITES TO TUMOR ANTIGENS

Award Information

Agency:
Department of Health and Human Services
Branch:
N/A
Award ID:
2980
Program Year/Program:
1987 / SBIR
Agency Tracking Number:
2980
Solicitation Year:
N/A
Solicitation Topic Code:
N/A
Solicitation Number:
N/A
Small Business Information
CREATIVE BIOMOLECULES, INC.
45 SOUTH STREET Hopkinton, MA 01748
View profile »
Woman-Owned: No
Minority-Owned: No
HUBZone-Owned: No
 
Phase 2
Fiscal Year: 1987
Title: ENGINEERING OF ANTIBODY BINDING SITES TO TUMOR ANTIGENS
Agency: HHS
Contract: N/A
Award Amount: $260,000.00
 

Abstract:

IN THIS RESEARCH, PROTEIN ENGINEERING IS BEING UTILIZED TO GENERATE THE MINIMAL COMBINING SITE OF ANTIBODIES, THE SO-CALLED FV, WHICH COMPRISES VH AND VL DOMAINS. IN PHASE I WE SYNTHESIZED GENES CODING FOR THE VH AND VL DOMAINS OF A SPECIFIC ANTIDIGOXIN ANTIBODY. THE BIOSYNTHETIC VH AND VL ARE BEING CLONED AND REASSEMBLED TO YIELD THE INTACT BINDING SITE. IN PHASE II, BASIC METHODOLOGY AND GENETIC ELEMENTS WILL BE DEVELOPED FOR PRODUCTION OF BIOSYNTHETIC ANTIBODY BINDING DOMAINS OF INTERCHANGEABLE SPECIFICITY, WHICH WILL ULTIMATELY BE DESIGNED TO BIND TUMOR ANTIGENS. VH AND VL GENES MADE FOR PHASE I WILL BE REFASHIONED SO THAT HYPERVARIABLE REGIONS WILL COPY THOSE OF AN ANTIPROTEIN ANTIBODY. THIS IS INTENDED TO ALTER SPECIFICITY WITHOUT THE NEED TO REMAKE CONSTANT FRAMEWORK OF THE FV. ATTEMPTS WILL BE MADE TO FUSE VH AND VL GENES INTO A SINGLE FV GENE VIA A LINKER WHICH WILL BE COMPATIBLE WITH NORMAL REFOLDING AND RECOMBINATION OF COMPONENT DOMAINS. THIS SINGLE POLYPEPTIDE CHAIN BINDING SITE WOULD BE USEFUL FOR IMMUNOTARGETING OF BIOLOGICALLY ACTIVE PROTEINS. FOR EXAMPLE, A SINGLE POLYPEPTIDE CHAIN COULD BE CLONED WHICH IS BIFUNCTIONAL, COMPRISING AN FV DOMAIN THAT BINDS A TUMOR ANTIGEN AND CYTOTOXIC DOMAIN THAT CONSISTS OF A RICIN A SUBUNIT. SUCH PROTEINS WOULD BE IDEAL THERAPEUTIC AGENTS FOR SELECTIVE CHEMOTHERAPY.

Principal Investigator:

James S. Huston
Principal Investigator
6174359001

Business Contact:

Small Business Information at Submission:

Creative Biomolecules, Inc.
35 South Street Hopkinton, MA 01748

EIN/Tax ID:
DUNS: N/A
Number of Employees: N/A
Woman-Owned: No
Minority-Owned: No
HUBZone-Owned: No