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Development of a method to multiplex ChIP-SEQ and ChIP-chip experiments

Award Information

Agency:
Department of Health and Human Services
Branch:
N/A
Award ID:
96281
Program Year/Program:
2010 / SBIR
Agency Tracking Number:
HG005282
Solicitation Year:
N/A
Solicitation Topic Code:
NHGRI
Solicitation Number:
N/A
Small Business Information
AFFOMIX, INC.
AFFOMIX, INC. 52 LEIGHTON TRAIL GUILFORD, CT 06437
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Woman-Owned: No
Minority-Owned: No
HUBZone-Owned: No
 
Phase 1
Fiscal Year: 2010
Title: Development of a method to multiplex ChIP-SEQ and ChIP-chip experiments
Agency: HHS
Contract: 1R43HG005282-01
Award Amount: $96,738.00
 

Abstract:

DESCRIPTION (provided by applicant): The immediate objective of our research is to generate a method that will enable researchers to multiplex Chromatin ImmunoPreciptation-Sequencing (ChIP-Seq) analysis in a single Next generation DNA sequencing run. In th e to-be-developed method: i) a set of antibodies directed against specific DNA-binding proteins are uniquely bar-coded with a DNA 'ZipCode;' ii) covalent cross-links between DNA-binding proteins and chromosomal DNA are formed by treating cells with formald ehyde; iii) the set of DNA-barcoded antibodies specific to the proteins of interest are used to selectively coimmunoprecipitate the protein-bound DNA fragments that were covalently cross-linked; iv) excess antibodies and chromosomal DNAs are removed by was hing; v) the enriched protein-bound chromosomal DNA is ligated to the antibody-attached ZipCode DNA using T4 DNA ligase, and; vi) the immunoprecipitated protein-DNA links are reversed and the recovered DNA is assayed using Next-generation sequencers to det ermine both the chromosomal DNA sequence bound by the protein and the antibody-identifying DNA ZipCode. PUBLIC HEALTH RELEVANCE: Researchers will benefit from an increased understanding of the function of human proteins. Methods are needed that can facilitate this understanding. The immediate objective of our research is to generate a method that will enable researchers to multiplex the method known as Chromatin ImmunoPreciptation-Sequencing (ChIP-Seq) analysis, in a single DNA sequencing run. We ant icipate using our high-throughput antibody-discovery pipeline for producing recombinant antibodies as a means to generate the reagents needed to make this multiplexed method possible.

Principal Investigator:

Michael P. Weiner
2034582844
MICHAEL@MWEINER.COM

Business Contact:


mweiner@affomix.com
Small Business Information at Submission:

AFFOMIX, INC.
AFFOMIX, INC. 688 East Main Street Branford, CT 06405

EIN/Tax ID: 184170700
DUNS: N/A
Number of Employees: N/A
Woman-Owned: No
Minority-Owned: No
HUBZone-Owned: No