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A bifunctional antibody screening system for both phage display and yeast…

Award Information

Agency:
Department of Health and Human Services
Branch:
N/A
Award ID:
95735
Program Year/Program:
2010 / SBIR
Agency Tracking Number:
AI085700
Solicitation Year:
N/A
Solicitation Topic Code:
NIAID
Solicitation Number:
N/A
Small Business Information
AFFOMIX, INC.
AFFOMIX, INC. 52 LEIGHTON TRAIL GUILFORD, CT 06437
View profile »
Woman-Owned: No
Minority-Owned: No
HUBZone-Owned: No
 
Phase 1
Fiscal Year: 2010
Title: A bifunctional antibody screening system for both phage display and yeast two-hyb
Agency: HHS
Contract: 1R43AI085700-01
Award Amount: $200,035.00
 

Abstract:

DESCRIPTION (provided by applicant): This is a proposal to establish a proof-of-principle for a vector system to isolate high-affinty antibodies through selective rounds in both yeast and Escherichia coli. Antibodies have quickly become extremely useful as therapeutic medicines to treat a wide-variety of disorders. We propose to develop a means of making the discovery of immunotherapeutics faster and cheaper. In this proposal we describe a system to isolate antigen-specific antibodies in yeast two-hybrid an d then quickly evolve them for both higher affinity and specificity using phage display. This new vector system will eliminate the need for any subcloning needed for protein expression, Y2H and phage display. The option for expressing and purifying antibod ies directly from E. coli or yeast is a direct application of the described system. The described system will allow for simultaneous testing of cDNA and antibody-antigen interactions in both yeast and E.coi-based systems. Completion of this project will fo rm the basis of a scalable system for a rapid and inexpensive means of affinity-maturation of recombinant antibodies screened in Y2H and increasing our understanding of protein- protein interactions. PUBLIC HEALTH RELEVANCE: Researchers will benefit from an increased understanding of the function of human proteins. Methods are needed that can facilitate this understanding. The immediate objective of our research is to generate a method that will enable researchers to multiplex the screening of affini ty reagents. We anticipate using our high-throughput pipeline for producing recombinant antibodies as a means to generate the reagents needed to make this method possible.

Principal Investigator:

Michael P. Weiner
2034582844
MICHAEL@MWEINER.COM

Business Contact:

Weiner
mweiner@affomix.com
Small Business Information at Submission:

AFFOMIX, INC.
AFFOMIX, INC. 688 East Main Street Branford, CT 06405

EIN/Tax ID: 184170700
DUNS: N/A
Number of Employees: N/A
Woman-Owned: No
Minority-Owned: No
HUBZone-Owned: No