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TOXIC CELLULAR DNA DAMAGE AND DISEASES

Award Information

Agency:
Department of Defense
Branch:
Army
Award ID:
3814
Program Year/Program:
1986 / SBIR
Agency Tracking Number:
3814
Solicitation Year:
N/A
Solicitation Topic Code:
N/A
Solicitation Number:
N/A
Small Business Information
Cytec Inc
Po Box 57462 Webster, TX 77598
View profile »
Woman-Owned: No
Minority-Owned: No
HUBZone-Owned: No
 
Phase 1
Fiscal Year: 1986
Title: TOXIC CELLULAR DNA DAMAGE AND DISEASES
Agency / Branch: DOD / ARMY
Contract: N/A
Award Amount: $49,910.00
 

Abstract:

THE OBJECTIVE OF THIS PHASE I SBIR PROJECT IS TO STUDY TECHNICAL/ METHODOLOGICAL ASPECTS, PERFORMANCE CRITERIA FOR ANEW CLINICAL SPECIMEN TOXICITY TEST. STATE-OF-THE-ART FLOW CYTOMETRIC/MONOCLONAL ANTIBODY TECHNOLOGY WOULD BE USED TO DETECT DNA DAMAGE, CYTOKINETIC ABNORMALITIES, AND ANEUPLOIDY. GENOTOXICITY AND CYTOKINETIC ABNORMALITIES WOULD BE ASSESSED IN VITRO BY LABELING WITH A HALOGENERATED PHRIMIDINE (IDURD) AND THEN USING MULTIPARAMETER FLOW CYTOMETRIC ANALYSIS OF DNA CONTENT AND THE AMOUNT OF IDURD INCORPORATED (DNA REPLICATION PATTERN) VIA A FLUORESCENT TAGGED MONOCLONAL ANTIBODY HIGHLY SPECIFIC FOR IDURD. WE PROPOSE TO DEVELOP: 1) AN IMMUNOTOXICITY TEST BASED ON SHORT-TERM CULTURE, BLASTOGENEIC RESPONSE TO PHA MITOGEN STIMULATED, PERIPHERAL BLOOD LYMPHOCYTES (ULTIMATELY T-& BCELL SUBSETS); AND 2) A HEPATOTOXICITY TEST USING IN VITRO IDURD LABELING OF FINE NEEDLE ASPIRATION BIOPSIES. PHASE I STUDIES WOULD UTILIZE DOMESTIC CANINES, WHEREAS BOTH CANINE AND HUMAN CLINICAL MATERIAL WOULD BE STUDIED IN PHASE II. THE HIGH TECHNOLOGY TEST(S) TO BE DEVELOPED WOULD BE VERSATILE; HAVE BROAD COMMERCIAL AND MILITARY APPLICATIONS; AND REPRESENT A MAJOR STEP FORWARD ANALYTICALLY FOR TOXICITY TEST DETECTION OF DNA DAMAGE, CELLULAR ABNORMALITIES, AND DISEASES ACQUIRED NATURALLY OR BY EXPOSURE TO BIOLOGICAL WEAPONS.

Principal Investigator:

Dr tod s johnson
7134844610

Business Contact:

Small Business Information at Submission:

Cytec Inc
Po Box 57462 Webster, TX 77598

EIN/Tax ID:
DUNS: N/A
Number of Employees:
Woman-Owned: No
Minority-Owned: No
HUBZone-Owned: No