USA flag logo/image

An Official Website of the United States Government

Tunable Source for Protein Fluorescence Lifetime Studies

Award Information

Agency:
Department of Health and Human Services
Branch:
N/A
Award ID:
70767
Program Year/Program:
2004 / SBIR
Agency Tracking Number:
RR018439
Solicitation Year:
N/A
Solicitation Topic Code:
N/A
Solicitation Number:
N/A
Small Business Information
DAKOTA TECHNOLOGIES, INC.
2201-A 12TH STREET NORTH FARGO, ND 58102
View profile »
Woman-Owned: No
Minority-Owned: No
HUBZone-Owned: No
 
Phase 1
Fiscal Year: 2004
Title: Tunable Source for Protein Fluorescence Lifetime Studies
Agency: HHS
Contract: 1R43RR018439-01A1
Award Amount: $175,301.00
 

Abstract:

DESCRIPTION (provided by applicant): Dakota Technologies, Inc. (DTI) has developed and demonstrated a low-cost and user-friendly method that generates fluorescence decay curves with exceptional speed, accuracy, and precision. Sample fluorescence is induced by a Nd:YAG microchip laser that operates at high repetition rate (5-10 kHz) and delivers sub-nanosecond pulses. Waveforms of the resulting fluorescence decay are directly recorded via a photomultiplier tube (PMT) and fast digitizer. The available excitation wavelengths are limited to the Nd:YAG harmonics at 532, 355, and 266 nm. Excitation sources that can be continuously tuned over a broad wavelength range and retain the high pulse repetition frequency and short pulse duration will be developed during this Phase I SBIR project. Particular emphasis will be placed on the 275-315 nm range that is optimal for fluorescence characterization of proteins. By perfecting these sources and integrating them into a commercial spectrofluorimeter, DTI will provide biochemists, biophysicists, and molecular biologists with a versatile, high performance tool for cutting edge research. A promising industrial application, verifying that proteins are produced in the appropriate conformation, has also been identified.

Principal Investigator:

Gregory D. Gillispie
7012374908
GILLISPIE@DAKOTATECHNOLOGIES.COM

Business Contact:

Gregory Gillispie
7012374908
GILLISPIE@DAKOTATECHNOLOGIES.COM
Small Business Information at Submission:

DAKOTA TECHNOLOGIES, INC.
2201-A 12TH STREET NORTH FARGO, ND 58102

EIN/Tax ID: 450432894
DUNS: N/A
Number of Employees: N/A
Woman-Owned: No
Minority-Owned: No
HUBZone-Owned: No