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Fluorescence Lifetime-Based Kinase Assay for HTS

Award Information

Agency:
Department of Health and Human Services
Branch:
N/A
Award ID:
80571
Program Year/Program:
2006 / SBIR
Agency Tracking Number:
GM074338
Solicitation Year:
N/A
Solicitation Topic Code:
N/A
Solicitation Number:
N/A
Small Business Information
DAKOTA TECHNOLOGIES, INC.
2201-A 12TH STREET NORTH FARGO, ND 58102
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Woman-Owned: No
Minority-Owned: No
HUBZone-Owned: No
 
Phase 1
Fiscal Year: 2006
Title: Fluorescence Lifetime-Based Kinase Assay for HTS
Agency: HHS
Contract: 1R43GM074338-01A1
Award Amount: $14,526.00
 

Abstract:

DESCRIPTION (provided by applicant): Fluorescence based-assays have greatly facilitated high-throughput screening (HTS) in drug discovery. However, in doing so, they created a bottleneck relating to false positives and false negatives, which may arise due to interferences from light scattering, inner filter effects, and compound fluorescence. Unlike fluorescence intensity, a fluorescence lifetime-based measurement is resistant to light scattering and inner filter effects, and provides the selectivity needed to discriminate the target fluorescence signal from background fluorescence. In Phase I we will demonstrate a homogenous fluorescence lifetime-based kinase assay that is resistant to fluorescence interferences and does not require the use of antibodies. Overcoming fluorescence interferences will cut costs associated with secondary screening and assist quicker identification of lead compounds. Furthermore, it will create an opportunity to screen compound libraries and/or natural products that are known to have some fluorescent properties, but were previously inaccessible by means of traditional fluorescence-based methods. Kinases are a major class of drug targets, which are involved in human diseases such as cancer, diabetes, and arthritis. This technology will advance the speed and efficiency in which we can find drugs to treat these important diseases.

Principal Investigator:

Kirk D. Hartel
7012374908
KHARTEL@DAKOTATECHNOLOGIES.COM

Business Contact:

Roxane Meidinger
7012374908
RMEDINGER@DAKOTATECHNOLOGIES.COM
Small Business Information at Submission:

DAKOTA TECHNOLOGIES, INC.
2201-A 12TH STREET NORTH FARGO, ND 58102

EIN/Tax ID: 450432894
DUNS: N/A
Number of Employees: N/A
Woman-Owned: No
Minority-Owned: No
HUBZone-Owned: No