USA flag logo/image

An Official Website of the United States Government

MAXIMUM LIKELIHOOD HIGH PERFORMANCE LIQUID CHROMATOGRAPHY MS/MS PROTEIN…

Award Information

Agency:
National Science Foundation
Branch:
N/A
Award ID:
17305
Program Year/Program:
1992 / SBIR
Agency Tracking Number:
17305
Solicitation Year:
N/A
Solicitation Topic Code:
N/A
Solicitation Number:
N/A
Small Business Information
Daniel H. Wagner, Associates, Incorporat
559 West Uwchlan Avenue Suite 140 Exton, PA -
View profile »
Woman-Owned: No
Minority-Owned: No
HUBZone-Owned: No
 
Phase 1
Fiscal Year: 1992
Title: MAXIMUM LIKELIHOOD HIGH PERFORMANCE LIQUID CHROMATOGRAPHY MS/MS PROTEIN SEQUENCING
Agency: NSF
Contract: N/A
Award Amount: $49,085.00
 

Abstract:

MANY PROBLEMS IN DISEASE REMEDIATION, CELL BIOLOGY, EVOLUTION, AND GENETICS REQUIRE COMPLETE KNOWLEDGE OF THE SEQUENCE OF AMINO ACIDS WHICH CONSTITUTE A PARTICULAR PROTEIN. EXISTING METHODS OF SEQUENCING SUCH AS EDMAN DEGRADATIONS REQUIRE SIGNIFICANT AMOUNTS OF PROTEIN MATERIAL AND LONG PERIODS OF TIME. USING CAPILLARY HIGH PERFORMANCE LIQUID CHORMATOGRAPHY (HPLC) AND TANDEM MASS SPECTROMETRY (MS/MS), RESEARCHERS HAVE DEMONSTRATED THE ABILITY TO SEQUENCE FEMTOMOLE QUANTITIES OF A SINGLE PROTEIN. ONE LIMITATION OF THIS TECHNIQUE IS THE PROBLEM OFRECONSTRUCTING THE PROTEIN SEQUENCE FROM THE MASS SPECTRUM. WE PROPOSE TO DEVELOP A MAXIMUM LIKELIHOOD RECONSTRUCTION ALGORITHM TO SUPPORT HPLC MS/MS PROTEIN SEQUENCING.

Principal Investigator:

Robert J. Lipshutz
4087451800

Business Contact:

Small Business Information at Submission:

Daniel H. Wagner Associates
894 Ross Dr Suite 205 Sunnyvale, CA 94089

EIN/Tax ID:
DUNS: N/A
Number of Employees:
Woman-Owned: No
Minority-Owned: No
HUBZone-Owned: No