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DEVELOPMENT OF A RAPID IMMUNOASSAY FOR SYPHILIS

Award Information

Agency:
Department of Health and Human Services
Branch:
N/A
Award ID:
7104
Program Year/Program:
1987 / SBIR
Agency Tracking Number:
7104
Solicitation Year:
N/A
Solicitation Topic Code:
N/A
Solicitation Number:
N/A
Small Business Information
Diamedix Corpon
2140 North Miami Avenue Miami, FL 33127
View profile »
Woman-Owned: No
Minority-Owned: No
HUBZone-Owned: No
 
Phase 1
Fiscal Year: 1987
Title: DEVELOPMENT OF A RAPID IMMUNOASSAY FOR SYPHILIS
Agency: HHS
Contract: N/A
Award Amount: $45,675.00
 

Abstract:

SYPHILIS, STILL A MAJOR SERIOUS INFECTIOUS DISEASE, CAN BE TREATED EFFECTIVELY IF DIAGNOSED EARLY. THE NUMBER OF DEATHS FROM SYPHILIS IN THE UNITED STATES HAS SHOWN A STEADY DECREASE, BUT THE NUMBER OF SYPHILIS CASES HAS NOT SHOWN A SIGNIFICANT DROP. SEROLOGICAL TESTS PLAY A MAJOR ROLE IN THE DIAGNOSIS OF SYPHILIS. THESE TESTS MUST BE PERFORMED BY QUALIFIED TECHNICIANS IN A LABORATORY. THE PROPOSED PROJECT IS TO DEVELOP A RAPID, SIMPLE, RELIABLE, AND INEXPENSIVE IMMUNOASSAY SUITABLE FOR USE IN PHYSICIANS' OFFICES. ANTIGENS OF THE CAUSATIVE AGENT TREPONEMA PALLIDUM WILL BE COATED ONTO A FILTER PAPER AS A SMALL DOT. IN THE ASSAY, THE TEST SERUM SAMPLE, ENZYME-LABELED ANTIBODIES TO HUMAN IMMUNOGLOBULINS, AND SUBSTRATE ARE ADDED SEQUENTIALLY TO THE ANTIGEN DOT. NO INCUBATION IS REQUIRED, SINCE THE SAMPLE AND REAGENTS DIFFUSE RAPIDLY, CONTACTING AND REACTING INSTANTANEOUSLY WITH EACH OTHER IN THE FIBER MESH. THE RESULTS ARE READ VISUALLY, AND A BLUE DOT INDICATES A POSITIVE REACTION. THE RESEARCH WILL FOCUS ON: (1) SELECTION OF FILTER PAPERS THAT WILL GIVE A MAXIMUM COATING OF ANTIGENS BUT A MINIMUM NONSPECIFIC ADSORPTION OF SERUM AND REAGENTS; (2) BEST CONDITIONS (TEMPERATURE, TIME, BUFFER, AND CONCENTRATION) FOR COATING ANTIGENS; (3) POSTCOATING (MATERIALS AND CONDITIONS) TO MINIMIZE OR ELIMINATE BACKGROUND REACTIONS; AND (4) VOLUME AND CONCENTRATION OF SAMPLE AND REAGENTS. A REFINED ASSAY PROCEDURE WILL BE TESTED WITH A PANEL OF MORE THAN 200 CLINICAL SERUM SAMPLES.

Principal Investigator:

Tsue-ming lin phd
3053242300

Business Contact:

Small Business Information at Submission:

Diamedix Corpon
2140 N Miami Avenue Miami, FL 33127

EIN/Tax ID:
DUNS: N/A
Number of Employees: N/A
Woman-Owned: No
Minority-Owned: No
HUBZone-Owned: No