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DETERMINATION OF A GENOTYPE INDEPENDENT METHOD OF MAIZE TRANSFORMATION

Award Information
Agency: Department of Agriculture
Branch: N/A
Contract: N/A
Agency Tracking Number: 10971
Amount: $48,401.00
Phase: Phase I
Program: SBIR
Solicitation Topic Code: N/A
Solicitation Number: N/A
Timeline
Solicitation Year: N/A
Award Year: 1989
Award Start Date (Proposal Award Date): N/A
Award End Date (Contract End Date): N/A
Small Business Information
6701 San Pablo Ave
Oakland, CA 94608
United States
DUNS: N/A
HUBZone Owned: No
Woman Owned: No
Socially and Economically Disadvantaged: No
Principal Investigator
 Karol E P Robinson
 () -
Business Contact
Phone: () -
Research Institution
N/A
Abstract

WE PROPOSE TO EXAMINE A METHOD OF GENOTYPE INDEPENDENT GENETIC TRANSFORMATION OF ZEA MAYS (MAIZE). THE DNA DELIVERY WILL BE ACCOMPLISHED USING HIGH-VELOCITY MICROPROJECTILES. THE FIRST STEP IN THE EXPERIMENT WILL BE TO TEST THE VARIOUS TISSUES OF MAIZE AS POTENTIAL TARGETS FOR THE INCORPORATION OF DNA. THE TISSUES CHOSEN WILL EACH BE CAPABLE OF TRANSMITTING INTEGRATED DNA TO THE NEXT GENERATION OF PLANTS AND WILL NOT REQUIRE ANY HIGHLY GENOTYPE-DEPENDENT TISSUE CULTURE. TO EVALUATE THEIR SUITABILITY, EACH TARGET TISSUE WILL BE BOMBARDED WITH MICROPROJECTILES CONTAINING A B-GLUCURONIDASE GENE SO THAT TRANSIENT EXPRESSION OF THE NEWLY DELIVERED DNA CAN BE MEASURED BY THE PRODUCTION OF BLUE COLOR. THESE TISSUES WILL THEN BE EVALUATED TO DETERMINE THE EFFECT OF DNA BOMBARDMENT ON THEIR NORMAL DEVELOPMENT. THE SECOND STEP IN THE EXPERIMENT WILL BE TO USE A COLOR MARKER AS A MEANS OF DETERMINING WHETHER STABLY TRANSFORMED TISSUES CAN BE OBTAINED FROM EACH TARGET TISSUE. THESE THREE PIECES OF INFORMATION (TRANSIENT EXPRESSION, TISSUE DEVELOPMENT, AND STABLE EXPRESSION) WILL ALLOW US TO DETERMINE THE OPTIMAL CONDITIONS FOR A GENOTYPE INDEPENDENT METHOD OF TRANSFORMATION OF MAIZE.

* Information listed above is at the time of submission. *

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