Soil Inhibition Resistant Mutants of Taq DNA Polymerase

Award Information

Agency: Department of Agriculture	Branch: N/A	Award ID: 72573	Program Year/Program: 2006 / SBIR
Agency Tracking Number: 2005-00194	Solicitation Year: N/A	Solicitation Topic Code: N/A	Solicitation Number: N/A

Small Business Information: DNA Polymerase Technology, Inc.
1508 S. Grand Blvd. Saint Louis, MO 63104-1364
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Woman-Owned: No

Minority-Owned: No

HUBZone-Owned: Yes

Phase 2: Fiscal Year: 2006

Title: Soil Inhibition Resistant Mutants of Taq DNA Polymerase

Agency: USDA

Contract: 2006-33610-17604

Award Amount: $290,400.00

Abstract:
Gene detection in soil using the Polymerase Chain Reaction (PCR) is nearly impossible due to the inhibitory affects of the components of soil, including humic acid. Currently, the DNA must be extracted from the soil in order to perform PCR. The DNA extraction/purification step is expensive and time consuming. We intend to develop commercially available PCR enzymes that will enable PCR to detect pathogens directly in soil, eliminating the extraction/purification step entirely.

Principal Investigator:
Milko B. Kermekchiev
3147715566
milko@klentaq.com

Business Contact:
Wayne M. Barnes
President
3146800575
wayne@klentaq.com

Small Business Information at Submission:

DNA Polymerase Technology, Inc.
1508 South Grand Blvd. St. Louis, MO 63104

EIN/Tax ID:

DUNS: N/A

Number of Employees: N/A

Woman-Owned: No

Minority-Owned: No

HUBZone-Owned: No