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Soil Inhibition Resistant Mutants of Taq DNA Polymerase

Award Information

Agency:
Department of Agriculture
Branch:
N/A
Award ID:
72573
Program Year/Program:
2006 / SBIR
Agency Tracking Number:
2005-00194
Solicitation Year:
N/A
Solicitation Topic Code:
N/A
Solicitation Number:
N/A
Small Business Information
DNA Polymerase Technology, Inc.
1508 S. Grand Blvd. Saint Louis, MO 63104-1364
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Woman-Owned: No
Minority-Owned: No
HUBZone-Owned: Yes
 
Phase 2
Fiscal Year: 2006
Title: Soil Inhibition Resistant Mutants of Taq DNA Polymerase
Agency: USDA
Contract: 2006-33610-17604
Award Amount: $290,400.00
 

Abstract:

Gene detection in soil using the Polymerase Chain Reaction (PCR) is nearly impossible due to the inhibitory affects of the components of soil, including humic acid. Currently, the DNA must be extracted from the soil in order to perform PCR. The DNA extraction/purification step is expensive and time consuming. We intend to develop commercially available PCR enzymes that will enable PCR to detect pathogens directly in soil, eliminating the extraction/purification step entirely.

Principal Investigator:

Milko B. Kermekchiev
3147715566
milko@klentaq.com

Business Contact:

Wayne M. Barnes
President
3146800575
wayne@klentaq.com
Small Business Information at Submission:

DNA Polymerase Technology, Inc.
1508 South Grand Blvd. St. Louis, MO 63104

EIN/Tax ID:
DUNS: N/A
Number of Employees: N/A
Woman-Owned: No
Minority-Owned: No
HUBZone-Owned: No