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Blood Inhibition Resistant Mutants of Taq DNA Polymerase

Award Information

Agency:
Department of Health and Human Services
Branch:
N/A
Award ID:
76282
Program Year/Program:
2005 / SBIR
Agency Tracking Number:
GM073401
Solicitation Year:
N/A
Solicitation Topic Code:
N/A
Solicitation Number:
N/A
Small Business Information
DNA Polymerase Technology, Inc.
1508 S. Grand Blvd. Saint Louis, MO 63104-1364
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Woman-Owned: No
Minority-Owned: No
HUBZone-Owned: Yes
 
Phase 1
Fiscal Year: 2005
Title: Blood Inhibition Resistant Mutants of Taq DNA Polymerase
Agency: HHS
Contract: 1R43GM073401-01
Award Amount: $107,144.00
 

Abstract:

DESCRIPTION (provided by applicant): The success and sensitivity of DNA detection is limited in important clinical, forensic, and environmental applications due to the presence of complex biological samples, such as blood, which reduce amplification efficiency. Different pre-PCR treatments that are being used to overcome this inhibitory effect in crude specimens significantly increase the duration and cost of the tests. We propose to develop and functionally characterize Taq polymerase mutants resistant to the blood inhibitory effect, which would allow a faster and lower-cost PCR analysis of crude samples. We also plan to obtain mutants of Taq with rapid DNA elongation, which will reduce the time of the amplification cycle, and we will attempt to add this feature to the blood-resistant phenotype. The screening for the above mutants will be performed by directed evolution of mutagenized Taq enzyme, using a high throughput version of this strategy, through which thousands of mutant clones are simultaneously amplified in individual micro-compartments of an emulsion PCR mixture. This proposal was motivated by our initial data showing that the two desired features of Taq, high resistance to blood inhibition and faster DNA elongation, are indeed obtainable by mutagenesis. Finally, in a potential phase two of the project, we will try to combine these two novel qualities of Taq with the cold-sensitive mutants of the enzyme designed for hot-start PCR which have already been developed and marketed by our company.

Principal Investigator:

Milko B. Kermekchiev
3147715566
MILKO@KLENTAQ.COM

Business Contact:

Wayne Barnes
3147715566
WAYNE@KLENTAQ.COM
Small Business Information at Submission:

Dna Polymerase Technology, Inc.
Dna Polymerase Technology, Inc. 1508 S Grand Blvd St. Louis, MO 63104

EIN/Tax ID: 431824012
DUNS: N/A
Number of Employees: N/A
Woman-Owned: No
Minority-Owned: No
HUBZone-Owned: No