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LIPOSOME OVERLAYS FOR PROTEIN-LIGAND SELECTIVITY

Award Information

Agency:
Department of Health and Human Services
Branch:
N/A
Award ID:
60991
Program Year/Program:
2002 / SBIR
Agency Tracking Number:
GM065683
Solicitation Year:
N/A
Solicitation Topic Code:
N/A
Solicitation Number:
N/A
Small Business Information
ECHELON BIOSCIENCES, INC.
675 ARAPEEN DRIVE, SUITE 302 SALT LAKE CITY, UT 84108-
View profile »
Woman-Owned: No
Minority-Owned: No
HUBZone-Owned: No
 
Phase 1
Fiscal Year: 2002
Title: LIPOSOME OVERLAYS FOR PROTEIN-LIGAND SELECTIVITY
Agency: HHS
Contract: 1R43GM065683-01
Award Amount: $100,000.00
 

Abstract:

Phosphoinositides (PIPns), e.g., phosphatidylinositol 4,5-bisphosphate (PI(4,5)P2), are key signaling molecules in cellular communication. Specific PIPns recruit target signaling proteins and intracellular adapter proteins to inner membrane sites to activate protein kinases and thereby initiate signal transduction cascades to regulate endocytosis, protein sorting, cell migration, and apoptosis. Determining the ligand selectivity of a given PIPn binding protein has become an important issue in deciphering downstream effectors, and in implementing drug discovery efforts for potential therapeutics that could control protein-phosphoinositide interactions. We will develop two lipid overlay systems to identify PIPn-specific lipid recognition proteins (LRPs). Both methods will identify proteins bound to a membrane. First we will prepare PIPn-containing liposomes to detect surface-bound LRPs. Second, we will extend this methodology to use stable polymerized liposomes. Then, the two lipid overlay systems will be provided to collaborators and potential customers for evaluation; the optimum composition will be manufactured for sale. Finally, these new tools will be used to develop commercial products.

Principal Investigator:

Colin G. Ferguson
8015880455
CFERGUSON@ECHELON-INC.COM

Business Contact:

John Wirthlin
8015880455
WWW.ECHELON-INC.COM
Small Business Information at Submission:

ECHELON BIOSCIENCES, INC.
ECHELON BIOSCIENCES, INC. 420 CHIPETA WAY, STE 180 SALT LAKE CITY, UT 84108

EIN/Tax ID: 841417180
DUNS: N/A
Number of Employees: N/A
Woman-Owned: No
Minority-Owned: No
HUBZone-Owned: No