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LUMINESCENCE DETECTION REAGENTS FOR PROTEINS

Award Information

Agency:
Department of Health and Human Services
Branch:
N/A
Award ID:
13882
Program Year/Program:
1990 / SBIR
Agency Tracking Number:
13882
Solicitation Year:
N/A
Solicitation Topic Code:
N/A
Solicitation Number:
N/A
Small Business Information
Ela Technologies Inc
Po Box 6033 Athens, GA 30604
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Woman-Owned: No
Minority-Owned: No
HUBZone-Owned: No
 
Phase 1
Fiscal Year: 1990
Title: LUMINESCENCE DETECTION REAGENTS FOR PROTEINS
Agency: HHS
Contract: N/A
Award Amount: $50,000.00
 

Abstract:

ELA TECHNOLOGIES, INC., HAS PRODUCED TWO BIOLUMINESCENT PROTEINS DESIGNATED RECAEQUORIN AND RECLUCIFERASE. THESE PROTEINS PRODUCE LIGHT WITH HIGH EFFICIENCY AND ARE DETECTABLE ON FILM IN THE PICOGRAM RANGE. RECAEQUORIN PRODUCES A FLASH OF LIGHT UPON THE ADDITION OF CALCIUM, AND RECLUCIFERASE PRODUCES A SUSTAINED SIGNAL IN THE PRESENCE OFITS SUBSTRATE, COELENTERATE LUCIFERIN. THIS RESEARCH WILL EVALUATE THE UTILITY OF THESE RECOMBINANT PROTEINS AS TAGS FOR THE DETECTION OF BIOTINYLATED PROTEINS AND PROTEIN ANTIGENS ON NITROCELLULOSE AND NYLON MEMBRANES USING BOTH INSTANT AND X-RAY FILM. THIS WILL BE ACCOMPLISHED BY COMPLETION OF THE FOLLOWING SPECIFIC AIMS: (1) TO USE BIOTINYLATED PROTEINS ON NITROCELLULOSE AND NYLON BLOTS AFTER TRANSFER FROM SLAB GEL ELECTROPHORETOGRAMS, (2) TO USE BIOTINYLATED RECAEQUORIN FORDETECTION OF BIOTINYLATED SECOND ANTIBODIES IN WESTERN BLOT TECHNIQUES, (3) TO PREPARE BIOTINYLATED RECLUCIFERASE AND EVALUATE THE USEFULNESS OF ITS SUSTAINED SIGNAL FOR APPLICATIONS DESCRIBED IN 1 AND 2, AND (4) TO PREPARE STREPTAVIDIN CONJUGATES OF BOTH RECAEQUORIN AND RECLUCIFERASE AS DIRECT SUBSTITUTES FOR THE COMMONLY USED ENZYME CONJUGATES SUCH AS AVIDIN/ALKALINE PHOSPHATASE.

Principal Investigator:

Nancy L Stults
4045469070

Business Contact:

Small Business Information at Submission:

Ela Technologies Inc
Po Box 6033 Athens, GA 30604

EIN/Tax ID:
DUNS: N/A
Number of Employees: N/A
Woman-Owned: No
Minority-Owned: No
HUBZone-Owned: No