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LUMINESCENCE DETECTION REAGENTS FOR PROTEINS

Award Information
Agency: Department of Health and Human Services
Branch: N/A
Contract: N/A
Agency Tracking Number: 13882
Amount: $50,000.00
Phase: Phase I
Program: SBIR
Solicitation Topic Code: N/A
Solicitation Number: N/A
Timeline
Solicitation Year: N/A
Award Year: 1990
Award Start Date (Proposal Award Date): N/A
Award End Date (Contract End Date): N/A
Small Business Information
Po Box 6033
Athens, GA 30604
United States
DUNS: N/A
HUBZone Owned: No
Woman Owned: No
Socially and Economically Disadvantaged: No
Principal Investigator
 Nancy L Stults
 (404) 546-9070
Business Contact
Phone: () -
Research Institution
N/A
Abstract

ELA TECHNOLOGIES, INC., HAS PRODUCED TWO BIOLUMINESCENT PROTEINS DESIGNATED RECAEQUORIN AND RECLUCIFERASE. THESE PROTEINS PRODUCE LIGHT WITH HIGH EFFICIENCY AND ARE DETECTABLE ON FILM IN THE PICOGRAM RANGE. RECAEQUORIN PRODUCES A FLASH OF LIGHT UPON THE ADDITION OF CALCIUM, AND RECLUCIFERASE PRODUCES A SUSTAINED SIGNAL IN THE PRESENCE OFITS SUBSTRATE, COELENTERATE LUCIFERIN. THIS RESEARCH WILL EVALUATE THE UTILITY OF THESE RECOMBINANT PROTEINS AS TAGS FOR THE DETECTION OF BIOTINYLATED PROTEINS AND PROTEIN ANTIGENS ON NITROCELLULOSE AND NYLON MEMBRANES USING BOTH INSTANT AND X-RAY FILM. THIS WILL BE ACCOMPLISHED BY COMPLETION OF THE FOLLOWING SPECIFIC AIMS: (1) TO USE BIOTINYLATED PROTEINS ON NITROCELLULOSE AND NYLON BLOTS AFTER TRANSFER FROM SLAB GEL ELECTROPHORETOGRAMS, (2) TO USE BIOTINYLATED RECAEQUORIN FORDETECTION OF BIOTINYLATED SECOND ANTIBODIES IN WESTERN BLOT TECHNIQUES, (3) TO PREPARE BIOTINYLATED RECLUCIFERASE AND EVALUATE THE USEFULNESS OF ITS SUSTAINED SIGNAL FOR APPLICATIONS DESCRIBED IN 1 AND 2, AND (4) TO PREPARE STREPTAVIDIN CONJUGATES OF BOTH RECAEQUORIN AND RECLUCIFERASE AS DIRECT SUBSTITUTES FOR THE COMMONLY USED ENZYME CONJUGATES SUCH AS AVIDIN/ALKALINE PHOSPHATASE.

* Information listed above is at the time of submission. *

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