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SBIR Phase I:In vivo Fluorescence Imaging Kit for Cell Proliferation.

Award Information

Agency:
National Science Foundation
Branch:
N/A
Award ID:
99035
Program Year/Program:
2010 / SBIR
Agency Tracking Number:
1013513
Solicitation Year:
N/A
Solicitation Topic Code:
BT1
Solicitation Number:
N/A
Small Business Information
MBMR
760 Parkside Avenue Suite 205 Brooklyn, NY 11226-0000
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Woman-Owned: No
Minority-Owned: No
HUBZone-Owned: No
 
Phase 1
Fiscal Year: 2010
Title: SBIR Phase I:In vivo Fluorescence Imaging Kit for Cell Proliferation.
Agency: NSF
Contract: 1013513
Award Amount: $149,996.00
 

Abstract:

This Small Business Innovation Research (SBIR) Phase I project is to apply novel bio-orthogonal tetrazine ligation technology to fluorescent labeling of DNA replication, and to ultimately produce a fluorescence imaging kit (FIK) for cell proliferation. The advantages are two-fold: 1) Because of the novel chemical mechanism, the FIK would allow for in vivo cell imaging, essentially providing continuous observation of cell proliferation with minimal interference from cell metabolism. Fluorescent microscopy could be done with live cells. 2) A single cell could be monitored through various stages of cell cycle. None of the existing methods allow for in vivo imaging of cell proliferation. Thus, conceptually, the difference between the capabilities of the existing assays and proposed FIK assay is analogous to the difference between a snapshot and a video recording. The broader impact/commercial potential of this project is to develop an assay for cell proliferation compatible with high-throughput screening technology. The assay can be applied to differentiate between quiescent and actively cycling stem cells, and, eventually, to differentiate between cancer stem cells and cancer cells. The low cost of reagents, simplicity of sample handling, and readiness to be adapted to a multi-well format make the proposed methodology an ideal candidate for application to high-throughput screening assays that will find applications in the fields of cancer biology and neurogenesis, as well as developmental and molecular biology. These fields all share a common research need for close observation of cell processes.

Principal Investigator:

Maksim Royzen
PhD
9179744391
royzen@gmail.com

Business Contact:

Maksim Royzen
PhD
9179744391
royzen@gmail.com
Small Business Information at Submission:

MBMR
2553 East 7th Street Apt 1C Brooklyn, NY 11235

EIN/Tax ID: 271204046
DUNS: N/A
Number of Employees:
Woman-Owned: No
Minority-Owned: No
HUBZone-Owned: No